Casp2^tm1Yuan
Targeted Allele Detail

Summary

• Symbol: Casp2^tm1Yuan
• Name: caspase 2; targeted mutation 1, Junying Yuan
• MGI ID: MGI:2159368
• Gene: Casp2 Location: Chr6:42241942-42259442 bp, + strand Genetic Position: Chr6, 20.54 cM
• Alliance: Casp2^tm1Yuan page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:47601
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin selection cassette replaced a 1.65 kb genomic fragment containing sequences that encode part of the active site of the protein and other essential regions. RT-PCR analysis on spleen mRNA derived from homozygous mice demonstrated that no detectable transcript is expressed from this allele, and western blot analysis on lysates derived from various tissues of homozygous mice confirmed that no detectable protein was encoded. (J:47601)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Casp2 Mutation: 22 strains or lines available

References

• Original: J:47601 Bergeron L, et al., Defects in regulation of apoptosis in caspase-2-deficient mice. Genes Dev. 1998 May 1;12(9):1304-14
• All: 17 reference(s)