Fadd^tm1Mak
Targeted Allele Detail

Summary

• Symbol: Fadd^tm1Mak
• Name: Fas associated via death domain; targeted mutation 1, Tak W Mak
• MGI ID: MGI:2159360
• Gene: Fadd Location: Chr7:144132060-144136178 bp, - strand Genetic Position: Chr7, 88.85 cM
• Alliance: Fadd^tm1Mak page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:46527
• Parent Cell Line: E14K (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin selection cassette replaced a genomic fragment containing the entire coding region of the gene. Western blot analysis on lysates derived from homozygous mice confirmed that no detectable protein was expressed from this allele. (J:46527)

Expression

In Mice Carrying this Mutation:	1 assay results
In Structures Affected by this Mutation:	3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Fadd Mutation: 19 strains or lines available

Notes

Authors in J:46527 state that injection of mutant ES cells into Gt(ROSA)26Sor E3.5 blastocysts (J:79478) resulting in high percentage chimeric embryos with a high contribution of mutant cells to the heart reproduce the phenotype of homozygous mutant embryos derived from heterozygous breedings. In contrast, chimeric embryos with a low contribution from the mutant ES cells display a normal phenotype at E11.5.

References

• Original: J:46527 Yeh WC, et al., FADD: essential for embryo development and signaling from some, but not all, inducers of apoptosis. Science. 1998 Mar 20;279(5358):1954-8
• All: 18 reference(s)