Thbd^tm1Wlr
Targeted Allele Detail

Summary

• Symbol: Thbd^tm1Wlr
• Name: thrombomodulin; targeted mutation 1, Harmut Weiler
• MGI ID: MGI:2158812
• Synonyms: Thbd^Pro, TM^P, TM^Pro
• Gene: Thbd Location: Chr2:148246391-148250108 bp, - strand Genetic Position: Chr2, 73.45 cM
• Alliance: Thbd^tm1Wlr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:47676
• Parent Cell Line: D3 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: Point mutations were introduced into the gene that altered codon 387 from one encoding glutamate to one endcoding proline. A neomycin selection cassette was also inserted downstream of the polyadenylation site in the 3' end of the gene. Immunohistochemistry experiments confirmed that a mutant protein was expressed from this allele in an identical pattern to the wild-type protein (data not shown). (J:47676)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Thbd Mutation: 26 strains or lines available

References

• Original: J:47676 Weiler-Guettler H, et al., A targeted point mutation in thrombomodulin generates viable mice with a prethrombotic state. J Clin Invest. 1998 May 1;101(9):1983-91
• All: 23 reference(s)