Chrne^tm1Vwi
Targeted Allele Detail

Summary

• Symbol: Chrne^tm1Vwi
• Name: cholinergic receptor, nicotinic, epsilon polypeptide; targeted mutation 1, Veit Witzemann
• MGI ID: MGI:2158741
• Synonyms: AChRepsilon -
• Gene: Chrne Location: Chr11:70505709-70510042 bp, - strand Genetic Position: Chr11, 43.14 cM
• Alliance: Chrne^tm1Vwi page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:36557
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A genomic fragment containing part of exon 7 and exon 8 was replaced with a neomycin selection cassette. These sequences encode essential regions for the function of the protien. RT-PCR experiments on RNA derived from muscle tissue of homozygous mice demonstrated that no detectable levels of wild-type transcript were produced from this allele. (J:36557)

Disease models

Expression

In Structures Affected by this Mutation:

5 anatomical structures

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Chrne Mutation: 19 strains or lines available

References

• Original: J:36557 Witzemann V, et al., Acetylcholine receptor epsilon-subunit deletion causes muscle weakness and atrophy in juvenile and adult mice. Proc Natl Acad Sci U S A. 1996 Nov 12;93(23):13286-91
• All: 4 reference(s)