Smad1^tm1Rjle
Targeted Allele Detail

Summary

• Symbol: Smad1^tm1Rjle
• Name: SMAD family member 1; targeted mutation 1, Robert J Lechleider
• MGI ID: MGI:2157982
• Gene: Smad1 Location: Chr8:80065024-80126057 bp, - strand Genetic Position: Chr8, 37.56 cM
• Alliance: Smad1^tm1Rjle page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:73279
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: Insertion of a PGK-neomycin resistance cassette into exon 3 disrupted the gene. RT-PCR analysis using primers that span the middle linker and MH2 domains did not detect transcript in homozygous mutant embryos. Western blot analysis did not detect any protein in homozygous mutant embryos. (J:73279)

Expression

In Mice Carrying this Mutation:	24 assay results
In Structures Affected by this Mutation:	7 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Smad1 Mutation: 32 strains or lines available

References

• Original: J:73279 Lechleider RJ, et al., Targeted mutagenesis of smad1 reveals an essential role in chorioallantoic fusion. Dev Biol. 2001 Dec 1;240(1):157-67
• All: 3 reference(s)