Itgam^tm1Bll
Targeted Allele Detail

Summary

• Symbol: Itgam^tm1Bll
• Name: integrin alpha M; targeted mutation 1, Christie M Ballantyne
• MGI ID: MGI:2157556
• Synonyms: CD11b-, CR3^-, CR3/MAC-1-, deltaalphaM, Mac-1-
• Gene: Itgam Location: Chr7:127661812-127717663 bp, + strand Genetic Position: Chr7, 69.93 cM, cytoband F4
• Alliance: Itgam^tm1Bll page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:39092
• Parent Cell Line: AB2.1 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1^b-m2

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A 0.7kb genomic fragment containing exon 4 was replaced with a neomycin selection cassette. RT-PCR and RNAse protection assays demonstrated that a mutant transcript is produced from this allele that splices exon 3 to exon 5, which results in a frameshift mutation. Immunohistochemistry analysis revealed that no encoded protein was detectable in spleen sections from homozygous mice. (J:39092)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Itgam Mutation: 47 strains or lines available

References

• Original: J:39092 Lu H, et al., LFA-1 is sufficient in mediating neutrophil emigration in Mac-1-deficient mice. J Clin Invest. 1997 Mar 15;99(6):1340-50
• All: 53 reference(s)