Lyn^tm1Ard
Targeted Allele Detail

Summary

• Symbol: Lyn^tm1Ard
• Name: LYN proto-oncogene, Src family tyrosine kinase; targeted mutation 1, Ashley R Dunn
• MGI ID: MGI:2157129
• Synonyms: Lyn-
• Gene: Lyn Location: Chr4:3676865-3791612 bp, + strand Genetic Position: Chr4, 2.05 cM
• Alliance: Lyn^tm1Ard page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:29356
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A PGK-neomycin resistance cassette replaced ~11.5kb of sequence, including the promoter and associated regulatory sequences. RT-PCR analysis of liver did not detect wild-type transcript in homozygous mutant mice. Immunoprecipitation and kinase assays of liver and spleen did not detect LYN autokinase activity in homozygous mutant mice. (J:29356)

Disease models

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 6 strains available Cell Lines: 0 lines available
• Carrying any Lyn Mutation: 64 strains or lines available

References

• Original: J:29356 Hibbs ML, et al., Multiple defects in the immune system of Lyn-deficient mice, culminating in autoimmune disease. Cell. 1995 Oct 20;83(2):301-11
• All: 37 reference(s)