Epha4^tm2Kln
Targeted Allele Detail

Summary

• Symbol: Epha4^tm2Kln
• Name: Eph receptor A4; targeted mutation 2, Rudiger Klein
• MGI ID: MGI:2156479
• Synonyms: ephA4^KD
• Gene: Epha4 Location: Chr1:77343822-77491725 bp, - strand Genetic Position: Chr1, 39.55 cM, cytoband C1-C5
• Alliance: Epha4^tm2Kln page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:67274
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Hypomorph, Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: A cDNA encoding a kinase dead mutant protein with a substitution of lysine 653 for methionine was fused in frame into exon 3 of the gene followed by a poly (A) site and a loxP flanked neomycin selection cassette. Western blot analysis confirmed the expression of the fusion protein in homozygous mice. (J:67274)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Epha4 Mutation: 68 strains or lines available

References

• Original: J:67274 Kullander K, et al., Kinase-dependent and kinase-independent functions of EphA4 receptors in major axon tract formation in vivo. Neuron. 2001 Jan;29(1):73-84
• All: 6 reference(s)