Chrm2^tm1Jwe
Targeted Allele Detail

Summary

• Symbol: Chrm2^tm1Jwe
• Name: cholinergic receptor, muscarinic 2, cardiac; targeted mutation 1, Jurgen Wess
• MGI ID: MGI:2155543
• Synonyms: M2^-, M2 KO, M2R^-, mAChR2^-
• Gene: Chrm2 Location: Chr6:36365019-36505349 bp, + strand Genetic Position: Chr6, 15.48 cM
• Alliance: Chrm2^tm1Jwe page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:53061
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A genomic fragment corresponding to sequences encoding the N terminus of the third transmemebrane region to the C terminus of the third intracellular loop was replaced with a neomycin resistance cassette. In situ hybridization experiments demonstrated that no transcript was detectable in brain tissue of homozygous mice. In addition, mutant hearts were unable to bind significant amounts of the muscarinic radioligand [3H]QNB, and muscarinic agonist-induced bradycardia was no longer observed. (J:53061)

Expression

In Structures Affected by this Mutation:

6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Chrm2 Mutation: 32 strains or lines available

References

• Original: J:53061 Gomeza J, et al., Pronounced pharmacologic deficits in M2 muscarinic acetylcholine receptor knockout mice. Proc Natl Acad Sci U S A. 1999 Feb 16;96(4):1692-7
• All: 60 reference(s)