App^tm1Cwe
Targeted Allele Detail

Summary

• Symbol: App^tm1Cwe
• Name: amyloid beta precursor protein; targeted mutation 1, Charles Weissmann
• MGI ID: MGI:2154522
• Synonyms: beta APP delta
• Gene: App Location: Chr16:84751236-84972187 bp, - strand Genetic Position: Chr16, 46.92 cM, cytoband C3-qter
• Alliance: App^tm1Cwe page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:21937
• Parent Cell Line: D3 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: A neomycin resistance cassette was inserted into exon 2 of the gene. Northern blots of brain, kidney, and lung tissues from homozygous mutant mice showed no detectable full-length wild-type mRNA. Two smaller bands were detectable by Northern blot. The authors attributed these bands to transcription through the inserted neomycin cassette and beyond. Further analysis by Western blot of brain extracts from homozygous mutant mice showed no full-length wild-type protein. Again, two bands, which the authors attributed to carboxy-terminal breakdown products of the shortened protein, were seen. The resulting proteins were determined to lack amino acids 20 - 75. (J:21937)

Expression

In Structures Affected by this Mutation:

6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any App Mutation: 89 strains or lines available

References

• Original: J:21937 Muller U, et al., Behavioral and anatomical deficits in mice homozygous for a modified beta-amyloid precursor protein gene. Cell. 1994 Dec 2;79(5):755-65
• All: 14 reference(s)