Col5a2^tm1Rmz
Targeted Allele Detail

Summary

• Symbol: Col5a2^tm1Rmz
• Name: collagen, type V, alpha 2; targeted mutation 1, Francesco Ramierez
• MGI ID: MGI:2152946
• Synonyms: pN
• Gene: Col5a2 Location: Chr1:45413491-45542442 bp, - strand Genetic Position: Chr1, 23.69 cM, cytoband C1
• Alliance: Col5a2^tm1Rmz page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:22301
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin resistance cassette replaced a genomic fragment containing exon 6. RT-PCR analysis revelaled that homozygous mice produce in-frame transcripts from this allele lacking exon 6 sequence. Western blot analysis identified a protein produced from this allele that presumably lacks the N-telopeptide segment of the alpha2(V) chain. (J:22301)

Expression

In Structures Affected by this Mutation:

6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Col5a2 Mutation: 67 strains or lines available

References

• Original: J:22301 Andrikopoulos K, et al., Targeted mutation in the col5a2 gene reveals a regulatory role for type V collagen during matrix assembly. Nat Genet. 1995 Jan;9(1):31-6
• All: 4 reference(s)