Fgf8^tm2Mrc
Targeted Allele Detail

Summary

• Symbol: Fgf8^tm2Mrc
• Name: fibroblast growth factor 8; targeted mutation 2, Mario R Capecchi
• MGI ID: MGI:2150351
• Synonyms: Fgf8^AP, Fgf8^C
• Gene: Fgf8 Location: Chr19:45725237-45731354 bp, - strand Genetic Position: Chr19, 38.75 cM, cytoband C3-D
• Alliance: Fgf8^tm2Mrc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:66265
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: LoxP sites were inserted flanking exon 5. An adjacent frt-flanked neomycin cassette was removed via Flp-mediated recombination in zygotes. An alkaline phosphatase gene was inserted just 5' to the second lox P site such that upon cre-mediated recombination, the alkaline phosphatase gene is expressed under the control of the Fgf8 gene. (J:66265)

Expression

In Mice Carrying this Mutation:	39 assay results
In Structures Affected by this Mutation:	16 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Fgf8 Mutation: 18 strains or lines available

References

• Original: J:66265 Moon AM, et al., Fgf8 is required for outgrowth and patterning of the limbs. Nat Genet. 2000 Dec;26(4):455-9
• All: 6 reference(s)