Calca^tm1Jpc
Targeted Allele Detail

Summary

• Symbol: Calca^tm1Jpc
• Name: calcitonin/calcitonin-related polypeptide, alpha; targeted mutation 1, Jean-Pierre Changeux
• MGI ID: MGI:2148771
• Synonyms: alphaCGRP -
• Gene: Calca Location: Chr7:114230713-114235592 bp, - strand Genetic Position: Chr7, 59.99 cM
• Alliance: Calca^tm1Jpc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:54422
• Parent Cell Line: HM-1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd-Hprt1^b-m3

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Two separate functional peptides, calcitonin and calcitonin gene related peptide-alpha (CGRP-alpha), are derived from this locus by alternative splicing. Calcitonin is encoded by exon 4 and CGRP-alpha by exon 5. Part of exon 5 was replaced with a lacZ gene and neomycin selection gene. Northern blot and RT-PCR analysis on samples derived from spinal root ganglia of homozygous mice demonstrated that no detectable CGRP-alpha transcript was produced from this allele. Western blot analysis on spinal ganglia extracts derived from homozygous mice confirmed that no detectable CGRP-alpha was made. Precursor calcitonin mRNA was detected in the spinal cord. (J:54422)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Calca Mutation: 21 strains or lines available

References

• Original: J:54422 Salmon AM, et al., Modulation of morphine analgesia in alphaCGRP mutant mice. Neuroreport. 1999 Mar 17;10(4):849-54
• All: 12 reference(s)