Prl^tm1Hmn
Targeted Allele Detail

Summary

• Symbol: Prl^tm1Hmn
• Name: prolactin; targeted mutation 1, Nelson D Horseman
• MGI ID: MGI:1934021
• Synonyms: PRL-
• Gene: Prl Location: Chr13:27241553-27249187 bp, + strand Genetic Position: Chr13, 12.25 cM
• Alliance: Prl^tm1Hmn page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:45243
• Parent Cell Line: D3 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: A PGK neomycin cassette was inserted into exon 4. This sequence encodes the second alpha helix of the protein. RT-PCR analysis confirmed a lack of wild-type transcript in homozygous mutant mice. Western blotting of pituitary gland extracts revealed the presence of a unique immunoreactive ~10-kDa truncated protein product in homozygous mutant mice. Disrupted prolactin synthesis was confirmed by biological assays. (J:45243)

Expression

In Structures Affected by this Mutation:

7 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Prl^tm1Hmn

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 4 strains available Cell Lines: 0 lines available
• Carrying any Prl Mutation: 28 strains or lines available

References

• Original: J:45243 Horseman ND, et al., Defective mammopoiesis, but normal hematopoiesis, in mice with a targeted disruption of the prolactin gene. EMBO J. 1997 Dec 1;16(23):6926-35
• All: 27 reference(s)