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Spp1tm1Rit
Targeted Allele Detail
Summary
Symbol: Spp1tm1Rit
Name: secreted phosphoprotein 1; targeted mutation 1, Susan R Rittling
MGI ID: MGI:1932084
Synonyms: Eta-1-, Opn-
Gene: Spp1  Location: Chr5:104582984-104588916 bp, + strand  Genetic Position: Chr5, 50.68 cM
Alliance: Spp1tm1Rit page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:56535
Parent Cell Line:  AB2.1 (ES Cell)
Strain of Origin:  129S7/SvEvBrd-Hprt1b-m2
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutation:    Insertion
 
Mutation detailsInsertion of a neomycin resistance cassette into exon 6 immediately 5' of the RGD sequence disrupted the Spp1 gene. Northern analysis of homozygous mutant kidney detected low levels of a high molecular weight RNA species, but no wild-type or truncated Spp1 transcript. Immunoblot analysis detected low levels of a truncated form of the protein in bone but not in other tissues, fluids, or cells of homozygous mutant mice. (J:56535)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Mice Carrying this Mutation: 2 assay results
In Structures Affected by this Mutation: 10 anatomical structure(s)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 6 strains available      Cell Lines: 0 lines available
Carrying any Spp1 Mutation:  40 strains or lines available
References
Original:  J:56535 Rittling SR, et al., Mice lacking osteopontin show normal development and bone structure but display altered osteoclast formation in vitro. J Bone Miner Res. 1998 Jul;13(7):1101-11
All:  95 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
05/28/2024
MGI 6.13
The Jackson Laboratory