Ltb^tm1Kpf
Targeted Allele Detail

Summary

• Symbol: Ltb^tm1Kpf
• Name: lymphotoxin B; targeted mutation 1, Klaus Pfeffer
• MGI ID: MGI:1860970
• Synonyms: LTbeta^-, LT beta delta
• Gene: Ltb Location: Chr17:35413483-35415281 bp, + strand Genetic Position: Chr17, 18.59 cM
• Alliance: Ltb^tm1Kpf page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:42508
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin resistance cassette flanked by loxP sites (floxed) replaced exon 3 of the gene. Mice carrying this allele were bred to mice that constitutively expressed cre-recombinase in all cells in early development (Schwenk et al., Nucleic Acids Res, 1995, 23:5080-5081). Resulting mice carrying the desired deletion were intercrossed to yield homozygous mutant mice at the desired locus. Northern blots of thymocytes and Con-A stimulated splenocytes from homozygous mutant mice showed no dectectable mRNA for the targeted gene. (J:42508)

Expression

In Mice Carrying this Mutation:	45 assay results
In Structures Affected by this Mutation:	15 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ltb Mutation: 18 strains or lines available

References

• Original: J:42508 Alimzhanov MB, et al., Abnormal development of secondary lymphoid tissues in lymphotoxin beta-deficient mice. Proc Natl Acad Sci U S A. 1997 Aug 19;94(17):9302-7
• All: 31 reference(s)