Parp1^tm1Jmdm
Targeted Allele Detail

Summary

• Symbol: Parp1^tm1Jmdm
• Name: poly (ADP-ribose) polymerase family, member 1; targeted mutation 1, Josiane Menissier de Murcia
• MGI ID: MGI:1857861
• Synonyms: PARP-, parp-1-, Parp-1 KO
• Gene: Parp1 Location: Chr1:180396456-180428564 bp, + strand Genetic Position: Chr1, 84.44 cM, cytoband H5
• Alliance: Parp1^tm1Jmdm page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:41742
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: A neomycin resistance cassette was inserted into the fourth exon of the gene. No ADPRT1 protein was present in homozygous mice as assayed by Western blot analysis and activity blot analysis using cells isolated from spleen and testis. (J:41742)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Parp1^tm1Jmdm

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Parp1 Mutation: 65 strains or lines available

Notes

With respect to apoptosis, the absence of ADRP drastically increased the sensitivity of primary bone marrow cells to apoptosis induced by an alkylating agent (J:54159). ADPRP deficient cell lines established from homozygous null animals show decreased topoisomerase II activity (J:54143) as well as decreased DNA polymerase activity (J:56869). NOTE: The similarities and differences between the Parp1^tm1Zqw and the Parp1^tm1Jmdm alleles are reviewed in (J:53842).

References

• Original: J:41742 de Murcia JM, et al., Requirement of poly(ADP-ribose) polymerase in recovery from DNA damage in mice and in cells. Proc Natl Acad Sci U S A. 1997 Jul 8;94(14):7303-7
• All: 53 reference(s)