Nos2^tm1Lau
Targeted Allele Detail

Summary

• Symbol: Nos2^tm1Lau
• Name: nitric oxide synthase 2, inducible; targeted mutation 1, Victor E Laubach
• MGI ID: MGI:1857228
• Synonyms: iNOS^-, iNOS-, iNOS KO, NOS2-, Nos2tm1Lau, NOS2^tm/Lau
• Gene: Nos2 Location: Chr11:78811613-78851052 bp, + strand Genetic Position: Chr11, 46.74 cM
• Alliance: Nos2^tm1Lau page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:29677
• Parent Cell Line: E14TG2a (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin cassette replaced exons 12 and 13 of the gene, which encode the calmodulin-binding domain. Northern and Western blots of IFNg/LPS-stimulated peritoneal macrophages showed no detectable Nos2 mRNA or protein, respectively. (J:29677, J:183641)

Disease models

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	22 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Nos2^tm1Lau

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 8 strains available Cell Lines: 0 lines available
• Carrying any Nos2 Mutation: 66 strains or lines available

References

• Original: J:29677 Laubach VE, et al., Mice lacking inducible nitric oxide synthase are not resistant to lipopolysaccharide-induced death. Proc Natl Acad Sci U S A. 1995 Nov 7;92(23):10688-92
• All: 491 reference(s)