Casp3 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:279716 Braitsch CM, et al., LATS1/2 suppress NFkappaB and aberrant EMT initiation to permit pancreatic progenitor differentiation. PLoS Biol. 2019 Jul;17(7):e3000382
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:8352886
Gene symbol:	Casp3
Gene name:	caspase 3

Antibody:	anti-cleaved Caspase-3 (Asp175) (#9661)
Detection system:	Secondary antibody coupled to Alexa Fluor

Results

S9E WT row 1 ©

Specimen S9E WT E11.5: embryonic day 11.5 (more

)
Note: Controls were either wild type or heterozygous littermates. Double labeled: green - Casp3; magenta - Cdh1 (assay MGI:8346384).

Structure	Level	Pattern	Image	Note
TS19: pancreas primordium epithelium	Weak	Single cells	S9E WT row 1	Few CASP3+ epithelial cells were observed in WT pancreas.

Specimen S9E Lats1/2^PanKO E11.5: embryonic day 11.5; Lats1^tm1.1Jfm/Lats1^tm1.1Jfm, Lats2^tm1.1Jfm/Lats2^tm1.1Jfm, Tg(Pdx1-cre)89.1Dam/0 (more

)
Note: Conditional mutant. Double labeled: green - Casp3; magenta - Cdh1 (assay MGI:8346384).

Structure	Level	Pattern	Image	Note
TS19: pancreas primordium epithelium	Weak	Single cells	S9E Lats1/2^PanKO row 1	There was no significant difference in the proportion of CASP3+ epithelial cells in Lats1/2<PanKO> pancreas at E11.5.

S9E WT row 2 ©

Specimen S9E WT E12.5: embryonic day 12.5 (more

)
Note: Controls were either wild type or heterozygous littermates. Double labeled: green - Casp3; magenta - Cdh1 (assay MGI:8346384).

Structure	Level	Pattern	Image	Note
TS20: pancreas epithelium	Weak	Single cells	S9E WT row 2	Few CASP3+ epithelial cells were observed in WT pancreas.

Specimen S9E Lats1/2^PanKO E12.5: embryonic day 12.5; Lats1^tm1.1Jfm/Lats1^tm1.1Jfm, Lats2^tm1.1Jfm/Lats2^tm1.1Jfm, Tg(Pdx1-cre)89.1Dam/0 (more

)
Note: Conditional mutant. Double labeled: green - Casp3; magenta - Cdh1 (assay MGI:8346384).

Structure	Level	Pattern	Image	Note
TS20: pancreas epithelium	Present	Single cells	S9E Lats1/2^PanKO row 2	Beginning at E12.5, the rate of cell death increased significantly in Lats1/2<PanKO> pancreas compared to wild type.

S9E WT row 3 ©

Specimen S9E WT E13.5: embryonic day 13.5 (more

)
Note: Controls were either wild type or heterozygous littermates. Double labeled: green - Casp3; magenta - Cdh1 (assay MGI:8346384).

Structure	Level	Pattern	Image	Note
TS21: pancreas epithelium	Weak	Single cells	S9E WT row 3	Few CASP3+ epithelial cells were observed in WT pancreas.

Specimen S9E Lats1/2^PanKO E13.5: embryonic day 13.5; Lats1^tm1.1Jfm/Lats1^tm1.1Jfm, Lats2^tm1.1Jfm/Lats2^tm1.1Jfm, Tg(Pdx1-cre)89.1Dam/0 (more

)
Note: Conditional mutant. Double labeled: green - Casp3; magenta - Cdh1 (assay MGI:8346384).

Structure	Level	Pattern	Image	Note
TS21: pancreas epithelium	Present	Single cells	S9E Lats1/2^PanKO row 3	The rate of cell death continued to increase in E13.5 Lats1/2<PanKO> pancreas compared to wild type.

S9E WT row 4 ©

Specimen S9E WT E14.5: embryonic day 14.5 (more

)
Note: Controls were either wild type or heterozygous littermates. Double labeled: green - Casp3; magenta - Cdh1 (assay MGI:8346384).

Structure	Level	Pattern	Image	Note
TS22: pancreas epithelium	Weak	Single cells	S9E WT row 4	Few CASP3+ epithelial cells were observed in WT pancreas.

Specimen S9E Lats1/2^PanKO E14.5: embryonic day 14.5; Lats1^tm1.1Jfm/Lats1^tm1.1Jfm, Lats2^tm1.1Jfm/Lats2^tm1.1Jfm, Tg(Pdx1-cre)89.1Dam/0 (more

)
Note: Conditional mutant. Double labeled: green - Casp3; magenta - Cdh1 (assay MGI:8346384).

Structure	Level	Pattern	Image	Note
TS22: pancreas epithelium	Present	Single cells	S9E Lats1/2^PanKO row 4	The rate of cell death continued to increase in E13.5 Lats1/2<PanKO> pancreas compared to wild type.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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