Pecam1 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:279716 Braitsch CM, et al., LATS1/2 suppress NFkappaB and aberrant EMT initiation to permit pancreatic progenitor differentiation. PLoS Biol. 2019 Jul;17(7):e3000382
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:8352259
Gene symbol:	Pecam1
Gene name:	platelet/endothelial cell adhesion molecule 1

Antibody:	anti-Pecam (MEC 13.3)
Detection system:	Secondary antibody coupled to Alexa Fluor

Results

Specimen S10A upper WT: embryonic day 13.5 (more

)
Note: Controls were either wild type or heterozygous littermates. Triple labeled: green - Pecam1; white - Cdh1 (assay MGI:8346384); red - pHH3.

Structure	Cell Type	Level	Pattern	Image	Note
TS21: pancreas	endothelial cell	Present		S10A WT upper

Specimen S10A upper Lats1/2^PanKO: embryonic day 13.5; Lats1^tm1.1Jfm/Lats1^tm1.1Jfm, Lats2^tm1.1Jfm/Lats2^tm1.1Jfm, Tg(Pdx1-cre)89.1Dam/0 (more

)
Note: Conditional mutant. Triple labeled: green - Pecam1; white - Cdh1 (assay MGI:8346384); red - pHH3.

Structure	Cell Type	Level	Pattern	Image	Note
TS21: pancreas	endothelial cell	Present		S10A Lats1/2^PanKO upper	Immunopositive endothelial cells failed to associate with Lats1/2<PanKO> Cdh1-positive cells.

Specimen S10A lower WT: embryonic day 13.5 (more

)
Note: Controls were either wild type or heterozygous littermates. Triple labeled: green - Pecam1; white - Cdh1 (assay MGI:8346384); red - pHH3.

Structure	Cell Type	Level	Pattern	Image	Note
TS21: pancreas	endothelial cell	Present		S10A WT lower

Specimen S10A lower Lats1/2^PanKO: embryonic day 13.5; Lats1^tm1.1Jfm/Lats1^tm1.1Jfm, Lats2^tm1.1Jfm/Lats2^tm1.1Jfm, Tg(Pdx1-cre)89.1Dam/0 (more

)
Note: Conditional mutant. Triple labeled: green - Pecam1; white - Cdh1 (assay MGI:8346384); red - pHH3.

Structure	Cell Type	Level	Pattern	Image	Note
TS21: pancreas	endothelial cell	Present		S10A Lats1/2^PanKO lower	Immunopositive endothelial cells failed to associate with Lats1/2<PanKO> Cdh1-positive cells.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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