Antibody:	TRAF7 Polyclonal antibody (11780-1-AP)
Detection system:	Secondary antibody coupled to Alexa Fluor 488

Structure	Level	Pattern	Image	Note
TS21: primitive collecting duct	Strong	Punctate	7a merge , 7a Traf7	Expressed on the basolateral and apical membranes.
TS21: ureteric tip	Strong	Punctate	7a merge , 7a Traf7	Expressed on the basolateral and apical membranes.
TS21: mesenchyme of metanephros	Weak	Diffuse	7a Traf7 , 7a merge	A mild diffuse signal was detected in the cytoplasm.
TS21: renal vesicle	Weak	Diffuse	7a Traf7 , 7a merge	A mild diffuse signal was detected in the cytoplasm.
TS21: early nephron	Weak	Diffuse	7a merge , 7a Traf7	A mild diffuse signal was detected in the cytoplasm of immature glomeruli.

Structure	Level	Pattern	Image	Note
TS21: primitive collecting duct	Strong	Punctate	7b merge , 7b Traf7	Expressed on the basolateral and apical membranes.
TS21: ureteric tip	Strong	Punctate	7b merge , 7b Traf7	Expressed on the basolateral and apical membranes.

Structure	Level	Pattern	Image	Note
TS21: primitive collecting duct	Strong	Punctate	7c merge , 7c Traf7	The staining pattern remained similar in the membrane.
TS21: ureteric tip	Strong	Punctate	7c merge , 7c Traf7	The staining pattern remained similar in the membrane.
TS21: early nephron	Present	Diffuse	7c Traf7 , 7c merge	Weaker diffuse cytoplasmic staining.
TS21: renal vesicle	Present	Diffuse	7c Traf7 , 7c merge	Weaker diffuse cytoplasmic staining.

Structure	Level	Pattern	Image	Note
TS21: primitive collecting duct	Present	Diffuse	7d merge , 7d Traf7	Weaker diffuse cytoplasmic staining.

Structure	Level	Pattern	Image	Note
TS28: glomerular tuft	Weak	Diffuse	8a merge , 8a Traf7	Mild diffuse cytoplasmic staining.
TS28: distal convoluted tubule	Moderate	Diffuse	8a merge , 8a Traf7
TS28: cortical collecting duct	Strong		8a Traf7 , 8a merge	A strong, granular signal was localized to the apical membranes.

Structure	Level	Pattern	Image	Note
TS28: loop of Henle thick limb	Moderate	Diffuse	8b Traf7 , 8b merge
TS28: medullary collecting duct	Weak		8b merge , 8b Traf7	mild staining
TS28: renal connecting tubule	Strong		8b Traf7 , 8b merge	Strong, granular staining in the apical membranes.
TS28: loop of Henle thin limb	Absent		8b Traf7 , 8b merge

Structure	Level	Pattern	Image	Note
TS28: distal convoluted tubule	Strong	Punctate	8c Traf7 , 8c merge	The staining intensity was elevated, showing randomly distributed strong punctate expression on the apical membrane.
TS28: glomerular tuft	Weak		8c Traf7 , 8c merge	The staining remained weak.
TS28: renal proximal convoluted tubule	Absent		8c Traf7 , 8c merge	Lacked detectable signals in the mutant.

Structure	Level	Pattern	Image	Note
TS28: loop of Henle thick limb	Strong		8d merge , 8d Traf7
TS28: loop of Henle thin limb	Absent		8d merge , 8d Traf7
TS28: medullary collecting duct	Absent		8d merge , 8d Traf7

Structure	Level	Pattern	Image	Note
TS28: glomerular tuft	Weak	Diffuse	9a Traf7 , 9a merge	Similar as that of P4.
TS28: distal convoluted tubule	Moderate	Diffuse	9a Traf7 , 9a merge	Similar as that of P4.
TS28: cortical collecting duct	Strong		9a Traf7 , 9a merge	Similar as that of P4.

Structure	Level	Pattern	Image	Note
TS28: loop of Henle thick limb	Moderate	Diffuse	9b merge , 9b Traf7	Similar as that of P4.
TS28: medullary collecting duct	Weak		9b merge , 9b Traf7	Similar as that of P4.
TS28: renal connecting tubule	Strong		9b merge , 9b Traf7	Similar as that of P4.
TS28: loop of Henle thin limb	Absent		9b Traf7 , 9b merge	Similar as that of P4.

Structure	Level	Pattern	Image	Note
TS28: renal proximal convoluted tubule	Strong	Punctate	9c merge , 9c Traf7	Strong punctate staining was also found on the apical membrane.
TS28: distal convoluted tubule	Strong	Punctate	9c Traf7 , 9c merge	Similar as that of P4.
TS28: glomerular tuft	Weak		9c Traf7 , 9c merge	Similar as that of P4.

Structure	Level	Pattern	Image	Note
TS28: loop of Henle thick limb	Present		9d Traf7 , 9d merge
TS28: loop of Henle thin limb	Present		9d merge , 9d Traf7	This was different from that of P4.
TS28: medullary collecting duct	Absent		9d merge , 9d Traf7	Similar as that of P4.

Structure	Level	Pattern	Image	Note
TS23: collecting duct	Strong	Punctate		Expressed on the basolateral and apical membranes.
TS23: ureteric tip	Strong	Punctate		Expressed on the basolateral and apical membranes.
TS23: mesenchyme of metanephros	Weak	Diffuse		A mild diffuse signal was detected in the cytoplasm.
TS23: renal vesicle	Weak	Diffuse		A mild diffuse signal was detected in the cytoplasm.
TS23: maturing glomerular tuft	Weak	Diffuse		A mild diffuse signal was detected in the cytoplasm of immature glomeruli.
TS23: cortical renal tubule	Present	Regionally restricted		Moderate to strong punctate cytoplasmic staining in the convoluted tubules.

Structure	Level	Pattern	Image	Note
TS23: collecting duct	Strong			The staining intensity was more pronounced in the mutant.
TS23: ureteric tip	Strong			The staining intensity was more pronounced in the mutant.
TS23: maturing glomerular tuft	Present			The staining intensity was more pronounced in the mutant.
TS23: mesenchyme of metanephros	Present			The staining intensity was more pronounced in the mutant.
TS23: renal vesicle	Present			The staining intensity was more pronounced in the mutant.
TS23: cortical renal tubule	Present	Regionally restricted		The staining intensity in the convoluted tubules was more pronounced in the mutant.

Structure	Level	Pattern	Image	Note
TS23: lung	Present		S3_merge lower , S3_Traf7