Antibody:	Anti-EDA2R/XEDAR (ab203667)
Detection system:	Secondary antibody coupled to Alexa Fluor 488

Structure	Cell Type	Level	Pattern	Image	Note
TS28: visceral epithelium of mature renal corpuscle		Moderate		2a Eda2r , 2a merge	visceral layer of Bowman's capsule
TS28: renal small blood vessel	blood vessel endothelial cell	Moderate	Scattered	2a Eda2r , 2a merge	Expressed sporadically in the vascular endothelium.
TS28: distal convoluted tubule		Weak	Diffuse	2a merge , 2a Eda2r	Expression was weak, diffuse, and cytoplasmic.
TS28: renal proximal convoluted tubule		Trace	Punctate	2a Eda2r , 2a merge	Expression was rare but, where present, appeared punctate and localized to either the apical or basal membrane of the tubules.

Structure	Level	Pattern	Image	Note
TS28: loop of Henle thick limb	Weak		2b Eda2r , 2b merge	Weak staining in the thick segments of the loop of Henle.
TS28: loop of Henle thin limb	Trace		2b Eda2r , 2b merge	Occasional positive cells in the thin segments.
TS28: medullary collecting duct	Trace		2b merge , 2b Eda2r	Scarce signal was detected in the cells of the conducting system, including the collecting ducts. Minimal co-localization with Aqp2 was detected.

Structure	Level	Pattern	Image	Note
TS28: distal convoluted tubule	Weak		2c Eda2r , 2c merge	Expression was weak, diffuse, and cytoplasmic, similar as in the wild-type.
TS28: renal proximal convoluted tubule	Trace		2c merge , 2c Eda2r	Expression was rare but, where present, appeared punctate and localized to either the apical or basal membrane of the tubules, similar as in the wild-type.

Structure	Level	Pattern	Image	Note
TS28: loop of Henle thick limb	Strong		2d merge , 2d Eda2r	Strong expression was detected in the mutant renal medulla, particularly in the thick segments of the loop of Henle. S statistically significant difference in expression between the cortex and medulla of the yotari phenotype, following a similar trend as in wild-type animals but without statistical significance.

Structure	Level	Pattern	Image	Note
TS28: renal cortex	Weak			Only minor variations between the cortex and medulla.
TS28: renal medulla	Weak			Only minor variations between the cortex and medulla.

Structure	Level	Pattern	Image	Note
TS28: renal cortex	Weak			Similar as in the wild-type. Only minor variations between the cortex and medulla.
TS28: renal medulla	Weak			Similar as in the wild-type. Only minor variations between the cortex and medulla.

Structure	Level	Pattern	Image	Note
TS23: skin	Present		S3_Eda2r , S3_merge upper