Antibody:	anti-LYVE-1
Detection system:	Secondary antibody coupled to Alexa Fluor
Assay notes:	The authors did not specify which antibody was used; MGI:8273612 may have been used instead.

Structure	Level	Pattern	Image	Note
TS28: diaphragm	Present	Regionally restricted	1C , 1A	Expression is in the submesothelial initial diaphragmatic lymphatic plexus.
TS28: lymphatic vessel	Present	Regionally restricted	1C , 1A	Expression is in the submesothelial initial diaphragmatic lymphatic plexus.

Structure	Level	Pattern	Image	Note
TS28: diaphragm	Present	Regionally restricted	1F , 1D	Expression is in the submesothelial initial diaphragmatic lymphatic plexus.
TS28: lymphatic vessel	Present	Regionally restricted	1F , 1D	Expression is in the submesothelial initial diaphragmatic lymphatic plexus.

Structure	Level	Pattern	Image	Note
TS24: liver	Present		1G E16.5
TS24: lymphatic vessel	Present	Regionally restricted	1G E16.5	Expression is in a few lymphatic vessels close to the thorax wall.

Structure	Level	Pattern	Image	Note
TS26: liver	Present		1G E18.5
TS26: lymphatic vessel	Present		1G E18.5	Expression is in lymphatic vessels close to the thorax wall.

Structure	Level	Pattern	Image	Note
TS27: lymphatic vessel	Present	Regionally restricted	1G P0	Expression is in lymphatic vessels that grew radially from the thorax wall toward the central tendon.

Structure	Level	Pattern	Image	Note
TS28: lymphatic vessel	Present	Regionally restricted	1G P5	Expression is in lymphatic vessels that grew radially from the thorax wall toward the central tendon.

Structure	Level	Pattern	Image	Note
TS28: lymphatic vessel	Present		1G P7	Expression is in the fully developed lymphatic vessel network with lymphatic vessels spanning from the thorax wall to the central tendon as individual or branched vessels.

Structure	Level	Pattern	Image	Note
TS27: lymphatic vessel	Present		1H P0	Diaphragmatic lymphatic vessel.

Structure	Level	Pattern	Image	Note
TS28: lymphatic vessel	Present		1H P5	Diaphragmatic lymphatic vessel.

Structure	Level	Pattern	Image	Note
TS28: lymphatic vessel	Present		1H P7 , 1I	Diaphragmatic lymphatic vessel.

Structure	Level	Pattern	Image	Note
TS28: lymphatic vessel	Present		1H 6W	Diaphragmatic lymphatic vessel.

Structure	Level	Pattern	Image	Note
TS28: lymphatic vessel	Present		2A	Diaphragmatic lymphatic vessel.

Structure	Level	Pattern	Image	Note
TS28: lymphatic vessel	Present	Regionally restricted	3A	Expression is in the lymphatic vessel plexus.
TS28: tail dermis	Present	Regionally restricted	3A	Expression is in the lymphatic vessel plexus.

Structure	Level	Pattern	Image	Note
TS28: lymphatic vessel	Present		4A

Structure	Level	Pattern	Image	Note
TS28: lymphatic vessel	Present		4C , 4B

Structure	Cell Type	Level	Pattern	Image	Note
TS22: diaphragm	macrophage	Present		4D

Structure	Cell Type	Level	Pattern	Image	Note
TS28: lymphatic vessel		Present		5A
TS28: diaphragm	macrophage	Present		5A

Structure	Level	Pattern	Image	Note
TS28: diaphragm	Present		5D
TS28: lymphatic vessel	Present		5D

Structure	Level	Pattern	Image	Note
TS28: diaphragm	Present		5E	This mutant had significantly increased lymphatic vessel branch counts per analyzed diaphragm segment.
TS28: lymphatic vessel	Present		5E	This mutant had significantly increased lymphatic vessel branch counts per analyzed diaphragm segment.

Structure	Level	Pattern	Image	Note
TS28: lymphatic vessel	Present		S1E
TS28: tail dermis	Present		S1E

Structure	Level	Pattern	Image	Note
TS28: lymphatic vessel	Present		S1F	This mutant had no significant differences in the positive area and the number of lymphatic vessel branches per lymphatic ring structure in the tail dermis compared to wild-type.
TS28: tail dermis	Present		S1F	This mutant had no significant differences in the positive area and the number of lymphatic vessel branches per lymphatic ring structure in the tail dermis compared to wild-type.