About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:361642 He Y, et al., HFM1 is essential for the germ cell intercellular bridge transport in primordial follicle formation in mice. Cell Mol Life Sci. 2024 Dec 27;82(1):28
Assay type: Immunohistochemistry
MGI Accession ID: MGI:8263047
Gene symbol: Ddx4
Gene name: DEAD box helicase 4
Antibody: Anti-DDX4/MVH (ab13840)
Detection system: Secondary antibody coupled to Alexa Fluor 594
Assay notes: Two Ddx4 antibodies were described, but the one used in this assay was not detailed. anti-DDX4 (ab27591) MGI:6690812 may have been used in this assay.
Results
Specimen 1c WT DDX4: embryonic day 17.5 (more )
Note: Double labeled: red - Ddx4; green - Hfm1 (assay MGI:8263046).
Structure Cell Type Level Pattern Image Note
TS25: ovary female germ cell Present 1c WT right , 1c WT left
Specimen 1c KO DDX4: embryonic day 17.5; Hfm1em1Gpt/Hfm1em1Gpt, Igs2em6(Gdf9-icre)Gpt/Igs2+ (more )
Note: Conditional mutant. Double labeled: red - Ddx4; green - Hfm1 (assay MGI:8263046).
Structure Cell Type Level Pattern Image Note
TS25: ovary female germ cell Weak 1c KO left , 1c KO right
Specimen 2c WT E17.5 DDX4: embryonic day 17.5 (more )
Note: Double labeled: red - Ddx4; green - Atp5f1a (assay MGI:8263052).
Structure Cell Type Level Pattern Image Note
TS25: ovary female germ cell Present 2c WT E17.5 right , 2c WT E17.5 left
Specimen 2c WT P1 DDX4: postnatal day 1 (more )
Note: Double labeled: red - Ddx4; green - Atp5f1a (assay MGI:8263052).
Structure Cell Type Level Pattern Image Note
TS27: ovary female germ cell Present 2c WT P1 left , 2c WT P1 right
Specimen 2c WT P3 DDX4: postnatal day 3 (more )
Note: Double labeled: red - Ddx4; green - Atp5f1a (assay MGI:8263052).
Structure Cell Type Level Pattern Image Note
TS27: ovary female germ cell Present 2c WT P3 right , 2c WT P3 left
Specimen 2c KO E17.5 DDX4: embryonic day 17.5; Hfm1em1Gpt/Hfm1em1Gpt, Igs2em6(Gdf9-icre)Gpt/Igs2+ (more )
Note: Conditional mutant. Double labeled: red - Ddx4; green - Atp5f1a (assay MGI:8263052).
Structure Level Pattern Image Note
TS25: ovary Present 2c KO E17.5 left , 2c KO E17.5 right
Specimen 2c KO P1 DDX4: postnatal day 1; Hfm1em1Gpt/Hfm1em1Gpt, Igs2em6(Gdf9-icre)Gpt/Igs2+ (more )
Note: Conditional mutant. Double labeled: red - Ddx4; green - Atp5f1a (assay MGI:8263052).
Structure Level Pattern Image Note
TS27: ovary Weak 2c KO P1 right , 2c KO P1 left
Specimen 2c KO P3 DDX4: postnatal day 3; Hfm1em1Gpt/Hfm1em1Gpt, Igs2em6(Gdf9-icre)Gpt/Igs2+ (more )
Note: Conditional mutant. Double labeled: red - Ddx4; green - Atp5f1a (assay MGI:8263052).
Structure Level Pattern Image Note
TS27: ovary Trace 2c KO P3 left , 2c KO P3 right
Specimen 3a WT E17.5 DDX4: embryonic day 17.5 (more )
Note: Double labeled: red - Ddx4; green - Pcnt (assay MGI:8263053).
Structure Level Pattern Image Note
TS25: ovary Present 3a WT E17.5 right , 3a WT E17.5 left
Specimen 3a WT P1 DDX4: postnatal day 1 (more )
Note: Double labeled: red - Ddx4; green - Pcnt (assay MGI:8263053).
Structure Cell Type Level Pattern Image Note
TS27: ovary female germ cell Present 3a WT P1 left , 3a WT P1 right
Specimen 3a WT P3 DDX4: postnatal day 3 (more )
Note: Double labeled: red - Ddx4; green - Pcnt (assay MGI:8263053).
Structure Cell Type Level Pattern Image Note
TS27: ovary female germ cell Present 3a WT P3 right , 3a WT P3 left
Specimen 3a KO E17.5 DDX4: embryonic day 17.5; Hfm1em1Gpt/Hfm1em1Gpt, Igs2em6(Gdf9-icre)Gpt/Igs2+ (more )
Note: Conditional mutant. Double labeled: red - Ddx4; green - Pcnt (assay MGI:8263053).
Structure Level Pattern Image Note
TS25: ovary Present 3a KO E17.5 right , 3a KO E17.5 left
Specimen 3a KO P1 DDX4: postnatal day 1; Hfm1em1Gpt/Hfm1em1Gpt, Igs2em6(Gdf9-icre)Gpt/Igs2+ (more )
Note: Conditional mutant. Double labeled: red - Ddx4; green - Pcnt (assay MGI:8263053).
Structure Level Pattern Image Note
TS27: ovary Present 3a KO P1 right , 3a KO P1 left
Specimen 3a KO P3 DDX4: postnatal day 3; Hfm1em1Gpt/Hfm1em1Gpt, Igs2em6(Gdf9-icre)Gpt/Igs2+ (more )
Note: Conditional mutant. Double labeled: red - Ddx4; green - Pcnt (assay MGI:8263053).
Structure Level Pattern Image Note
TS27: ovary Present 3a KO P3 right , 3a KO P3 left
Specimen 5a WT E17.5 DDX4: embryonic day 17.5 (more )
Note: Double labeled: red - Ddx4; green - Tubg1 (assay MGI:8263056).
Structure Cell Type Level Pattern Image Note
TS25: ovary female germ cell Present 5a WT E17.5 right , 5a WT E17.5 left
Specimen 5a WT P1 DDX4: postnatal day 1 (more )
Note: Double labeled: red - Ddx4; green - Tubg1 (assay MGI:8263056).
Structure Cell Type Level Pattern Image Note
TS27: ovary female germ cell Present 5a WT P1 right , 5a WT P1 left
Specimen 5a WT P3 DDX4: postnatal day 3 (more )
Note: Double labeled: red - Ddx4; green - Tubg1 (assay MGI:8263056).
Structure Cell Type Level Pattern Image Note
TS27: ovary female germ cell Present 5a WT P3 left , 5a WT P3 right
Specimen 5a KO E17.5 DDX4: embryonic day 17.5; Hfm1em1Gpt/Hfm1em1Gpt, Igs2em6(Gdf9-icre)Gpt/Igs2+ (more )
Note: Conditional mutant. Double labeled: red - Ddx4; green - Tubg1 (assay MGI:8263056).
Structure Level Pattern Image Note
TS25: ovary Weak 5a KO E17.5 right , 5a KO E17.5 left
Specimen 5a KO P1 DDX4: postnatal day 1; Hfm1em1Gpt/Hfm1em1Gpt, Igs2em6(Gdf9-icre)Gpt/Igs2+ (more )
Note: Conditional mutant. Double labeled: red - Ddx4; green - Tubg1 (assay MGI:8263056).
Structure Level Pattern Image Note
TS27: ovary Weak 5a KO P1 right , 5a KO P1 left
Specimen 5a KO P3 DDX4: postnatal day 3; Hfm1em1Gpt/Hfm1em1Gpt, Igs2em6(Gdf9-icre)Gpt/Igs2+ (more )
Note: Conditional mutant. Double labeled: red - Ddx4; green - Tubg1 (assay MGI:8263056).
Structure Level Pattern Image Note
TS27: ovary Trace 5a KO P3 right , 5a KO P3 left
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
09/15/2026
MGI 6.29 		The Jackson Laboratory