Antibody:	anti-spectrin beta V
Detection system:	Secondary antibody

Structure	Cell Type	Level	Pattern	Image	Note
TS28: organ of Corti	auditory hair cell	Present		1E beta V	Expression in sensory hair cells only.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: organ of Corti	cochlear outer hair cell	Weak		1E inset	Strong expression in outer hair cells.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: organ of Corti	auditory hair cell	Present	Regionally restricted	1F beta V upper left	Expression restricted to cortical lattice, along cell lateral wall.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: organ of Corti	auditory hair cell	Present	Regionally restricted	1F beta V upper right	Expression restricted to cortical lattice, along cell lateral wall.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: organ of Corti	auditory hair cell	Present	Regionally restricted	1F beta V lower left	Expression restricted to cortical lattice, along cell lateral wall.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: organ of Corti	auditory hair cell	Present	Regionally restricted	1F beta V lower right	Expression restricted to cortical lattice, along cell lateral wall.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: organ of Corti	auditory hair cell	Present		2D left	Expression restricted to hair cells.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: organ of Corti	auditory hair cell	Present		2D right	Expression restricted to hair cells.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: organ of Corti	cochlear inner hair cell	Present	Regionally restricted	2E left	In isolated inner hair cells, expression is cytoplasmic, with strong submembrane expression at neck region.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: organ of Corti	cochlear inner hair cell	Present	Regionally restricted	2E middle	In isolated inner hair cells, expression is cytoplasmic, with strong submembrane expression at neck region.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: organ of Corti	cochlear inner hair cell	Present	Regionally restricted	2E right	In isolated inner hair cells, expression is cytoplasmic, with strong submembrane expression at neck region.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: organ of Corti	cochlear outer hair cell	Present	Regionally restricted	2G beta V , 2G beta V + F-actin	In isolated outer hair cells, expression extends all along lateral wall.

Structure	Cell Type	Level	Pattern	Image	Note
TS26: organ of Corti	auditory hair cell	Present		S2A beta V	Expression only in auditory hair cells.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: organ of Corti	auditory hair cell	Present		S2B beta V + tubulin	Expression only in auditory hair cells.

Structure	Level	Pattern	Image	Note
TS28: stria vascularis	Present	Regionally restricted	S4C beta V , S4C + F-actin	Expresion in vessels within and nearby stria vascularis epithelium.

Structure	Cell Type	Level	Pattern	Image	Note
TS22: cochlear duct	endothelial cell	Strong	Regionally restricted	S4D beta V left , S4D beta V right	Strong expression in endothelial cells of inner ear.

Structure	Level	Pattern	Image	Note
TS28: cochlea	Present	Regionally restricted	S4E beta V	Expression in endothelial cell in region beneath cochlear spiral limbus.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: organ of Corti	cochlear outer hair cell	Present	Regionally restricted	S5A upper middle	Expression in outer hair cell lateral wall. No expression in apical and basal synaptic zone of hair cells.
TS28: organ of Corti	organ of Corti supporting cell	Absent		S5A upper middle

Structure	Cell Type	Level	Pattern	Image	Note
TS28: organ of Corti	cochlear outer hair cell	Present	Regionally restricted	S5A upper right
TS28: organ of Corti	organ of Corti supporting cell	Absent		S5A upper right

Structure	Cell Type	Level	Pattern	Image	Note
TS28: organ of Corti	cochlear outer hair cell	Present	Regionally restricted	S5A lower left	Expression in outer hair cell lateral wall. No expression in apical and basal synaptic zone of hair cells.
TS28: organ of Corti	organ of Corti supporting cell	Absent		S5A lower left

Structure	Cell Type	Level	Pattern	Image	Note
TS28: organ of Corti	cochlear outer hair cell	Present	Regionally restricted	S5A lower middle	Expression in outer hair cell lateral wall. No expression in apical and basal synaptic zone of hair cells.
TS28: organ of Corti	organ of Corti supporting cell	Absent		S5A lower middle

Structure	Cell Type	Level	Pattern	Image	Note
TS28: organ of Corti	cochlear outer hair cell	Present	Regionally restricted	S5A lower right	Expression in outer hair cell lateral wall. No expression in apical and basal synaptic zone of hair cells.
TS28: organ of Corti	organ of Corti supporting cell	Absent		S5A lower right

Structure	Cell Type	Level	Pattern	Image	Note
TS28: organ of Corti	cochlear inner hair cell	Present	Regionally restricted	S5B upper left	Differences in intensity of expression between inner hair cells and outer hair cells.
TS28: organ of Corti	cochlear outer hair cell	Present	Regionally restricted	S5B upper left	Differences in intensity of expression between inner hair cells and outer hair cells.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: organ of Corti	cochlear inner hair cell	Present	Regionally restricted	S5B upper right	Differences in intensity of expression between inner hair cells and outer hair cells.
TS28: organ of Corti	cochlear outer hair cell	Present	Regionally restricted	S5B upper right	Differences in intensity of expression between inner hair cells and outer hair cells.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: organ of Corti	cochlear inner hair cell	Present	Regionally restricted	S5B lower left	Differences in intensity of expression between inner hair cells and outer hair cells.
TS28: organ of Corti	cochlear outer hair cell	Present	Regionally restricted	S5B lower left	Differences in intensity of expression between inner hair cells and outer hair cells.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: organ of Corti	cochlear inner hair cell	Present	Regionally restricted	S5B lower right	Differences in intensity of expression between inner hair cells and outer hair cells.
TS28: organ of Corti	cochlear outer hair cell	Present	Regionally restricted	S5B lower right	Differences in intensity of expression between inner hair cells and outer hair cells.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: organ of Corti	cochlear outer hair cell	Present	Regionally restricted	S6A OHC 1	Expression in entire lateral wall of outer hair cells, irrespective of their position in the cochlea

Structure	Cell Type	Level	Pattern	Image	Note
TS28: organ of Corti	cochlear outer hair cell	Present	Regionally restricted	S6A OHC 2	Expression in entire lateral wall of outer hair cells, irrespective of their position in the cochlea

Structure	Cell Type	Level	Pattern	Image	Note
TS28: organ of Corti	cochlear outer hair cell	Present	Regionally restricted	S6A OHC 3	Expression in entire lateral wall of outer hair cells, irrespective of their position in the cochlea

Structure	Cell Type	Level	Pattern	Image	Note
TS28: organ of Corti	cochlear outer hair cell	Present	Regionally restricted	S6A OHC 4	Expression in entire lateral wall of outer hair cells, irrespective of their position in the cochlea

Structure	Cell Type	Level	Pattern	Image	Note
TS28: organ of Corti	cochlear outer hair cell	Present	Regionally restricted	S6A OHC 5	Expression in entire lateral wall of outer hair cells, irrespective of their position in the cochlea

Structure	Cell Type	Level	Pattern	Image	Note
TS28: organ of Corti	cochlear outer hair cell	Present	Regionally restricted	S6A OHC 6	Expression in entire lateral wall of outer hair cells, irrespective of their position in the cochlea

Structure	Cell Type	Level	Pattern	Image	Note
TS28: organ of Corti	cochlear inner hair cell	Present	Regionally restricted	S6A IHC 1	Typical 'ring' pattern of expression only in apical neck region of inner hair cells.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: organ of Corti	cochlear inner hair cell	Present	Regionally restricted	S6A IHC 2	Typical 'ring' pattern of expression only in apical neck region of inner hair cells.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: organ of Corti	cochlear inner hair cell	Present	Regionally restricted	S6A IHC 3	Typical 'ring' pattern of expression only in apical neck region of inner hair cells.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: organ of Corti	cochlear inner hair cell	Present	Regionally restricted	S6A IHC 4	Typical 'ring' pattern of expression only in apical neck region of inner hair cells.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: organ of Corti	cochlear inner hair cell	Present	Regionally restricted	S6A IHC 5	Typical 'ring' pattern of expression only in apical neck region of inner hair cells.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: organ of Corti	cochlear inner hair cell	Present	Regionally restricted	S6A IHC 6	Typical 'ring' pattern of expression only in apical neck region of inner hair cells.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: organ of Corti	cochlear outer hair cell	Present	Regionally restricted	S6B beta V , S6B merged (right)	Expression along lateral wall of outer hair cells.

Structure	Cell Type	Level	Pattern	Image	Note
TS27: organ of Corti	cochlear outer hair cell	Trace		5C P2 basal	Expression in outer hair cells increases from P2 to P10.

Structure	Cell Type	Level	Pattern	Image	Note
TS27: organ of Corti	cochlear outer hair cell	Trace		5C P2 middle	Expression in outer hair cells increases from P2 to P10.

Structure	Cell Type	Level	Pattern	Image	Note
TS27: organ of Corti	cochlear outer hair cell	Trace		5C P2 apical	Expression in outer hair cells increases from P2 to P10.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: organ of Corti	cochlear outer hair cell	Weak		5C P4 basal	Expression in outer hair cells increases from P2 to P10.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: organ of Corti	cochlear outer hair cell	Present		5C P4 middle	Expression in outer hair cells increases from P2 to P10.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: organ of Corti	cochlear outer hair cell	Weak		5C P4 apical	Expression in outer hair cells increases from P2 to P10.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: organ of Corti	cochlear outer hair cell	Present		5C P8 basal	Expression in outer hair cells increases from P2 to P10.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: organ of Corti	cochlear outer hair cell	Present		5C P8 middle	Expression in outer hair cells increases from P2 to P10.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: organ of Corti	cochlear outer hair cell	Present		5C P8 apical	Expression in outer hair cells increases from P2 to P10.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: organ of Corti	cochlear outer hair cell	Strong		5C P10 basal	Expression in outer hair cells increases from P2 to P10.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: organ of Corti	cochlear outer hair cell	Strong		5C P10 middle	Expression in outer hair cells increases from P2 to P10.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: organ of Corti	cochlear outer hair cell	Strong		5C P10 apical	Expression in outer hair cells increases from P2 to P10.

Structure	Cell Type	Level	Pattern	Image	Note
TS27: organ of Corti	cochlear outer hair cell	Present	Regionally restricted	6B beta V base	In outer hair cells, diffuse expression within cytoplasm.

Structure	Cell Type	Level	Pattern	Image	Note
TS27: organ of Corti	cochlear outer hair cell	Present	Regionally restricted	6B beta V apex	In outer hair cells, diffuse expression within cytoplasm.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: organ of Corti	cochlear outer hair cell	Present	Regionally restricted	6C beta V left	In outer hair cells, expression in forming cortical lattice.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: organ of Corti	cochlear outer hair cell	Present	Regionally restricted	6C beta V right	In outer hair cells, expression in forming cortical lattice.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: organ of Corti	cochlear outer hair cell	Present	Regionally restricted	6D beta V	Expression exetnds entire length of outer hair cell lateral wall.