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Gene Expression Data
Assay Details
Assay
Reference: J:363289 Cao D, et al., Selective utilization of glucose metabolism guides mammalian gastrulation. Nature. 2024 Oct;634(8035):919-928
Assay type: Immunohistochemistry
MGI Accession ID: MGI:8169065
Gene symbol: T
Gene name: brachyury, T-box transcription factor T
Antibody: anti-Brachyury (AF2085)
Detection system: Secondary antibody coupled to Alexa Fluor
Assay notes: Two T antibodies were described, but the one used in this assay was not detailed. Anti-Brachyury (81694S) MGI:8168929 may have been used in this assay.
Results
Specimen ED2a T/BRA: embryonic day 6.75 (more )
Note: Mid streak stage. Double labeled: teal - T; pseudocolor - Slc2a1 (assay MGI:8169061).
Structure Level Pattern Image Note
TS10: primitive streak Present ED2a Primitive streak , ED2a GLUT1 T/BRA
Specimen ED2d T/BRA: embryonic day 7.25 (more )
Note: Late streak stage. Double labeled: yellow - T; teal - Foxa2 (assay MGI:8169069).
Structure Level Pattern Image Note
TS10: primitive streak Present ED2d FOXA2 T/BRA
Specimen ED6b T/BRA (upper): embryonic day 6.25 (more )
Note: Early streak stage. Double labeled: purple - T; pseudocolor - Slc2a1 (assay MGI:8169061).
Structure Level Pattern Image Note
TS9: primitive streak Present ED6b Anterior GLUT1 T/BRA , ED6b Anterior GLUT1 T/BRA inset
Specimen ED6b T/BRA (lower): embryonic day 6.25 (more )
Note: Early streak stage. Double labeled: purple - T; pseudocolor - Slc2a1 (assay MGI:8169061).
Structure Level Pattern Image Note
TS9: primitive streak Present ED6b Posterior GLUT1 T/BRA inset , ED6b Posterior GLUT1 T/BRA
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory