Neurog2 In situ reporter (knock in) Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:174488 Britz O, et al., A role for proneural genes in the maturation of cortical progenitor cells. Cereb Cortex. 2006 Jul;16 Suppl 1:i138-51
Assay type:	In situ reporter (knock in)
MGI Accession ID:	MGI:7790520
Gene symbol:	Neurog2
Gene name:	neurogenin 2

Reporter:	GFP
Antibody:	anti-GFP
Detection system:	Secondary antibody coupled to Alexa Fluor 488

Results

Specimen 2K: embryonic day 15.5; Neurog2^tm4Fgu/Neurog2⁺ (more

)
Note: Embryos were exposed to one 2 hours prior to sacrifice and a second BrdU 30 minutes prior to harvesting to label S/G2/M-phase progenitors. Double labeled: green - Neurog2; red - BrdU.

Structure	Level	Pattern	Image	Note
TS23: cerebral cortex ventricular layer	Present		2K	GFP-expressing cells were for the most part BrdU negative (9.5% double positive vs. 31.2% expected if GFP were expressed in S-phase).
TS23: cerebral cortex subventricular zone	Present		2K	The vast majority cells were GFP positive.

Specimen 2M: embryonic day 15.5; Neurog2^tm4Fgu/Neurog2⁺ (more

)
Note: Double labeled: green - Neurog2 reporter; red - Neurog2 protein (assay MGI:7790494).

Structure	Level	Pattern	Image	Note
TS23: cerebral cortex ventricular layer	Present		2M	GFP was largely coexpressed with Neurog2.
TS23: cerebral cortex subventricular zone	Present		2M	The vast majority cells were GFP positive.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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