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Gene Expression Data
Assay Details
Assay
Reference: J:174488 Britz O, et al., A role for proneural genes in the maturation of cortical progenitor cells. Cereb Cortex. 2006 Jul;16 Suppl 1:i138-51
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7790504
Gene symbol: Neurog2
Gene name: neurogenin 2
Antibody: Anti-Ngn2
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 1J: embryonic day 12.5 (more )
Note: Double labeled: green - Neurog2; red - Neurog1 (assay MGI:7790493).
Structure Level Pattern Image Note
TS20: cerebral cortex ventricular layer Present 1J Coexpressed with Neurog1.
Specimen 2A: embryonic day 15.5 (more )
Note: Embryos were exposed to BrdU 30 minutes prior to harvesting to label S-phase cells. Double labeled: green - Neurog2; red - BrdU.
Structure Level Pattern Image Note
TS23: cerebral cortex ventricular layer Present 2A The percentage of Neurog2/BrdU double-positive progenitors was only 5.4% of the total Neurog2+ population, a fraction well below the proportion of positive progenitors expected to be in S-phase if Neurog2 was homogeneously expressed throughout the cell cycle (25.4%).
Specimen 2B: embryonic day 15.5 (more )
Note: Embryos were exposed to BrdU 8 hours prior to harvesting to label early-to-mid G1 cells. Double labeled: green - Neurog2; red - BrdU.
Structure Level Pattern Image Note
TS23: cerebral cortex ventricular layer Present 2B 43.9% of the positive nuclei incorporated BrdU, similar to the proportion of 49.6% expected if Neurog2 was randomly expressed throughout the cell cycle.
Specimen 2C: embryonic day 15.5 (more )
Note: Embryos were exposed to BrdU 14 hours prior to harvesting to label late G1 cells. Double labeled: green - Neurog2; red - BrdU.
Structure Level Pattern Image Note
TS23: cerebral cortex ventricular layer Present 2C A significantly larger fraction of positive cohort colabeled with BrdU (70.3%) as compared with that expected from a random distribution of Neurog2+ nuclei through the cell cycle (50.9%).
Specimen 2D: embryonic day 15.5 (more )
Note: Double labeled: green - Neurog2; red - pHH3.
Structure Level Pattern Image Note
TS23: cerebral cortex ventricular layer Present 2D Only occasional positive nuclei were colabeled by anti-pHH3.
Specimen 2L: embryonic day 15.5; Neurog2tm4Fgu/Neurog2+ (more )
Note: Embryos were exposed to one 2 hours prior to sacrifice and a second BrdU 30 minutes prior to harvesting to label S/G2/M-phase progenitors. Double labeled: green - Neurog2; red - BrdU.
Structure Level Pattern Image Note
TS23: cerebral cortex ventricular layer Present 2L The percentage of Neurog2/BrdU double-positive progenitors was only 10% of the total Neurog2+ population, compared with an expected 36.4% if Neurog2 was homogeneously expressed throughout the cell cycle.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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12/30/2025
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