Nefm Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:174095 Cai Z, et al., Loss of shp2-mediated mitogen-activated protein kinase signaling in muller glial cells results in retinal degeneration. Mol Cell Biol. 2011 Jul;31(14):2973-83
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7788469
Gene symbol:	Nefm
Gene name:	neurofilament, medium polypeptide

Antibody:

Results

Specimen 2G WT: postnatal day 21 (more

)
Note: Double labeled: red - Nefm; green - Pax6 (assay MGI:7788451).

Structure	Level	Pattern	Image	Note
TS28: retina layer	Present		2G WT
TS28: retina nerve fiber layer	Present		2G WT

Specimen 2G cKO: postnatal day 21; Ptpn11^tm1Gsf/Ptpn11^tm1Gsf, Tg(Six3-cre)69Frty/0 (more

)
Note: Conditional mutant. Double labeled: red - Nefm; green - Pax6 (assay MGI:7788451).

Structure	Level	Pattern	Image	Note
TS28: retina layer	Present	Regionally restricted	2G cKO	Reduced expression compared with the control. The positive nerve fibers in the optic nerve fiber layer/inner limiting membrane layer were lost.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 09/30/2025 MGI 6.24