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Gene Expression Data
Assay Details
Assay
Reference: J:298035 Kelly NH, et al., Single cell RNA-sequencing reveals cellular heterogeneity and trajectories of lineage specification during murine embryonic limb development. Matrix Biol. 2020 Jul;89:1-10
Assay type: RNA in situ
MGI Accession ID: MGI:7738312
Gene symbol: Col2a1
Gene name: collagen, type II, alpha 1
Probe: Col2a1 probe
Probe preparation: Antisense RNA
Assay notes: A grayscale version of the individual channels is provided in Supplemental Fig. 4.
Results
Specimen 4 E11.5: embryonic day 11.5 (more )
Note: Triple labeled: green - Col2a1; white - Krt14 (assay MGI:7738313); magenta - Myog (assay MGI:7738314).
Structure Level Pattern Image Note
TS19: footplate mesenchyme Present Regionally restricted 4E11.5 Col2a1 , 4E11.5 Merge Expressed across a diffuse area, which coalesced into cartilaginous rays indicating the future sites of bones.
Specimen 4 E13.5: embryonic day 13.5 (more )
Note: Triple labeled: green - Col2a1; white - Krt14 (assay MGI:7738313); magenta - Myog (assay MGI:7738314).
Structure Level Pattern Image Note
TS21: hindlimb cartilage condensation Present 4E13.5 Merge , 4E13.5 Col2a1 Expressed in cartilaginous rays.
Specimen 4 E15.5: embryonic day 15.5 (more )
Note: Triple labeled: green - Col2a1; white - Krt14 (assay MGI:7738313); magenta - Myog (assay MGI:7738314).
Structure Level Pattern Image Note
TS23: hindlimb cartilage condensation Present Regionally restricted 4E15.5 Merge , 4E15.5 Col2a1 Expression in the cartilaginous rays were now interrupted, indicating joint cavitation had occurred.
Specimen 4 E18.5: embryonic day 18.5 (more )
Note: Triple labeled: green - Col2a1; white - Krt14 (assay MGI:7738313); magenta - Myog (assay MGI:7738314).
Structure Level Pattern Image Note
TS26: hindlimb cartilage condensation Present Regionally restricted 4E18.5 Col2a1 , 4E18.5 Merge Expression in the cartilaginous rays were now interrupted, indicating joint cavitation had occurred.
TS26: foot joint Present Regionally restricted 4E18.5 Col2a1 Expressed in the joint surfaces.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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