Pou5f1 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:279207 Mager J, A Catalog of Early Lethal KOMP Phenotypes. MGI Direct Data Submission. 2019-2024;
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7736338
Gene symbol:	Pou5f1
Gene name:	POU domain, class 5, transcription factor 1

Antibody:	anti-Oct4 (ab27985)
Detection system:	Secondary antibody coupled to Alexa Fluor 647

Results

Taf13_merged (upper) ©

Taf13_Oct4 (upper) ©

Specimen +/+ or +/-: embryonic day 6.5 (more

)
Note: Littermate control; genotype may be either +/+ or +/-. Multi-labeled: white - Pou5f1; cyan - Cdh1 (assay MGI:7736335); yellow - Cdx2 (assay MGI:7736336); magenta - Nanog (assay MGI:7736337); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS8: epiblast	Present		Taf13_Oct4 (upper) , Taf13_merged (upper)

Specimen -/-: embryonic day 6.5; Taf13^em1(IMPC)J/Taf13^em1(IMPC)J (more

)
Note: Multi-labeled: white - Pou5f1; cyan - Cdh1 (assay MGI:7736335); yellow - Cdx2 (assay MGI:7736336); magenta - Nanog (assay MGI:7736337); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS8: epiblast	Absent		Taf13_merged (lower) , Taf13_Oct4 (lower)

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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