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Gene Expression Data
Assay Details
Assay
Reference: J:306217 Lee H, et al., Cell cycle-related kinase is a crucial regulator for ciliogenesis and Hedgehog signaling in embryonic mouse lung development. BMB Rep. 2020 Jul;53(7):367-372
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7719991
Gene symbol: Tubg1
Gene name: tubulin, gamma 1
Antibody: anti-gamma tubulin
Detection system: Secondary antibody coupled to Alexa Fluor 594
Assay notes: The specificity of the antibody used was not detailed; both family members have been annotated.
Results
Specimen 3E WT upper: embryonic day 14.5 (more )
Note: Double labeled: red - Tubg1 and Tubg2; green - Arl13b (assay MGI:7719989).
Structure Cell Type Level Pattern Image Note
TS22: lung mesenchyme lung multiciliated epithelial cell Present 3E WT upper Expressed in the basal bodies.
Specimen 3E KO upper: embryonic day 14.5; Cdk20tm1.1Jegg/Cdk20tm1.1Jegg (more )
Note: Double labeled: red - Tubg1 and Tubg2; green - Arl13b (assay MGI:7719989).
Structure Cell Type Level Pattern Image Note
TS22: lung epithelium lung multiciliated epithelial cell Present 3E KO upper Expressed in the basal bodies. Decreased ciliary frequency compared with the wild-type.
Specimen 3E WT lower: embryonic day 14.5 (more )
Note: Double labeled: red - Tubg1 and Tubg2; green - Arl13b (assay MGI:7719989).
Structure Cell Type Level Pattern Image Note
TS22: lung mesenchyme lung multiciliated epithelial cell Present 3E WT lower Expressed in the basal bodies.
Specimen 3E KO lower: embryonic day 14.5; Cdk20tm1.1Jegg/Cdk20tm1.1Jegg (more )
Note: Double labeled: red - Tubg1 and Tubg2; green - Arl13b (assay MGI:7719989).
Structure Cell Type Level Pattern Image Note
TS22: lung mesenchyme lung multiciliated epithelial cell Present 3E KO lower Expressed in the basal bodies. Decreased ciliary frequency compared with the wild-type.
Specimen S3E: embryonic day 17.5 (more )
Note: Double labeled: red - Tubg1 and Tubg2; green - Arl13b (assay MGI:7719989).
Structure Cell Type Level Pattern Image Note
TS25: lung epithelium multiciliated columnar cell of tracheobronchial tree Present S3E Expressed in the basal bodies.
Specimen S3F: embryonic day 17.5; Cdk20tm1.1Jegg/Cdk20tm1.1Jegg (more )
Note: Double labeled: red - Tubg1 and Tubg2; green - Arl13b (assay MGI:7719989).
Structure Cell Type Level Pattern Image Note
TS25: lung epithelium multiciliated columnar cell of tracheobronchial tree Present S3F Expressed in the basal bodies. Decreased ciliary frequency compared with the wild-type.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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