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Gene Expression Data
Assay Details
Assay
Reference: J:306217 Lee H, et al., Cell cycle-related kinase is a crucial regulator for ciliogenesis and Hedgehog signaling in embryonic mouse lung development. BMB Rep. 2020 Jul;53(7):367-372
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7719961
Gene symbol: Sox9
Gene name: SRY (sex determining region Y)-box 9
Antibody: anti-Sox9
Detection system: Secondary antibody coupled to Alexa Fluor 594
Results
Specimen 2A WT: embryonic day 12.5 (more )
Note: Double labeled: red - Sox9; green - Sox2 (assay MGI:7719953).
Structure Level Pattern Image Note
TS20: lung epithelium Present Regionally restricted 2A WT Expressed in the distal regions.
Specimen 2A KO: embryonic day 12.5; Cdk20tm1.1Jegg/Cdk20tm1.1Jegg (more )
Note: Double labeled: red - Sox9; green - Sox2 (assay MGI:7719953).
Structure Level Pattern Image Note
TS20: lung epithelium Present Regionally restricted 2A KO Overall expression patterns were not changed, but bud growth and branching morphogenesis was markedly reduced.
Specimen 2B WT E14.5 upper: embryonic day 14.5 (more )
Note: Double labeled: red - Sox9; green - Sox2 (assay MGI:7719953).
Structure Level Pattern Image Note
TS22: bronchiole epithelium Present 2B WT E14.5 upper
Specimen 2B WT E14.5 lower: embryonic day 14.5 (more )
Note: Double labeled: red - Sox9; green - Sox2 (assay MGI:7719953).
Structure Level Pattern Image Note
TS22: bronchiole epithelium Present 2B WT E14.5 lower
Specimen 2B KO E14.5 upper: embryonic day 14.5; Cdk20tm1.1Jegg/Cdk20tm1.1Jegg (more )
Note: Double labeled: red - Sox9; green - Sox2 (assay MGI:7719953).
Structure Level Pattern Image Note
TS22: bronchiole epithelium Present 2B KO E14.5 upper Mutant lungs exhibited relatively increased early bud stages and decreased flattened and bifurcated cleft stages than in the wild-type controls.
Specimen 2B KO E14.5 lower: embryonic day 14.5; Cdk20tm1.1Jegg/Cdk20tm1.1Jegg (more )
Note: Double labeled: red - Sox9; green - Sox2 (assay MGI:7719953).
Structure Level Pattern Image Note
TS22: bronchiole epithelium Present 2B KO E14.5 lower Mutant lungs exhibited relatively increased early bud stages and decreased flattened and bifurcated cleft stages than in the wild-type controls.
Specimen 2B WT E16.5 upper: embryonic day 16.5 (more )
Note: Double labeled: red - Sox9; green - Sox2 (assay MGI:7719953).
Structure Level Pattern Image Note
TS24: bronchiole epithelium Present 2B WT E16.5 upper
Specimen 2B WT E16.5 lower: embryonic day 16.5 (more )
Note: Double labeled: red - Sox9; green - Sox2 (assay MGI:7719953).
Structure Level Pattern Image Note
TS24: bronchiole epithelium Present 2B WT E16.5 lower
Specimen 2B KO E16.5 upper: embryonic day 16.5; Cdk20tm1.1Jegg/Cdk20tm1.1Jegg (more )
Note: Double labeled: red - Sox9; green - Sox2 (assay MGI:7719953).
Structure Level Pattern Image Note
TS24: bronchiole epithelium Present 2B KO E16.5 upper Mutant lungs exhibited relatively increased early bud stages and decreased flattened and bifurcated cleft stages than in the wild-type controls.
Specimen 2B KO E16.5 lower: embryonic day 16.5; Cdk20tm1.1Jegg/Cdk20tm1.1Jegg (more )
Note: Double labeled: red - Sox9; green - Sox2 (assay MGI:7719953).
Structure Level Pattern Image Note
TS24: bronchiole epithelium Present 2B KO E16.5 lower Mutant lungs exhibited relatively increased early bud stages and decreased flattened and bifurcated cleft stages than in the wild-type controls.
Specimen 2D WT E14.5 middle: embryonic day 14.5 (more )
Note: Double labeled: red - Sox9; green - EdU.
Structure Level Pattern Image Note
TS22: bronchiole epithelium Present 2D WT E14.5 middle
Specimen 2D KO E14.5 middle: embryonic day 14.5; Cdk20tm1.1Jegg/Cdk20tm1.1Jegg (more )
Note: Double labeled: red - Sox9; green - EdU.
Structure Level Pattern Image Note
TS22: bronchiole epithelium Present 2D KO E14.5 middle EdU-labeled distal epithelial cells were significantly diminished.
Specimen 2D WT E16.5 middle: embryonic day 16.5 (more )
Note: Double labeled: red - Sox9; green - EdU.
Structure Level Pattern Image Note
TS24: bronchiole epithelium Present 2D WT E16.5 middle
Specimen 2D KO E16.5 middle: embryonic day 16.5; Cdk20tm1.1Jegg/Cdk20tm1.1Jegg (more )
Note: Double labeled: red - Sox9; green - EdU.
Structure Level Pattern Image Note
TS24: bronchiole epithelium Present 2D KO E16.5 middle EdU-labeled distal epithelial cells were significantly diminished.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/24/2026
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