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Gene Expression Data
Assay Details
Assay
Reference: J:352718 Gorla M, et al., A Nedd4 E3 Ubiquitin Ligase Pathway Inhibits Robo1 Repulsion and Promotes Commissural Axon Guidance across the Midline. J Neurosci. 2022 Oct 5;42(40):7547-7561
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7716477
Gene symbol: Robo3
Gene name: roundabout guidance receptor 3
Antibody: Anti-Robo3 (AF3076)
Detection system: Secondary antibody coupled to Cy3
Results
Specimen 6D: embryonic day 11.5 (more )
Note: Double labeled: red - Robo3; green - Nedd4 (assay MGI:7716475).
Structure Level Pattern Image Note
TS20: spinal cord Present Regionally restricted 6D Merge , 6D Robo3 Expression was detected in commissural axons and co-localized with Nedd4.
Specimen 6E: embryonic day 11.5 (more )
Note: Double labeled: red - Robo3; green - Nedd4l (assay MGI:7716476).
Structure Level Pattern Image Note
TS20: spinal cord Present Regionally restricted 6E Robo3 , 6E Merge Expression was detected in commissural axons and co-localized with Nedd4l.
Specimen 9A Control top: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS20: spinal cord Present Regionally restricted 9A Control bottom Robo3 is specifically expressed in all populations of dorsal commissural axons as they project toward and across the floor plate midline.
Specimen 9A Control bottom: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS20: spinal cord Present Regionally restricted 9A Control top Robo3 is specifically expressed in all populations of dorsal commissural axons as they project toward and across the floor plate midline.
Specimen 9B Control: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: spinal cord Present Regionally restricted 9B Control Robo3 is specifically expressed in all populations of dorsal commissural axons as they project toward and across the floor plate midline.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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12/30/2025
MGI 6.24 		The Jackson Laboratory