Gene Expression Data
Assay Details
Assay
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Reference:
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J:46667
Fode C, et al., The bHLH protein NEUROGENIN 2 is a determination factor for epibranchial placode-derived sensory neurons. Neuron. 1998 Mar;20(3):483-94
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7666577
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Gene symbol:
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Neurog2
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Gene name:
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neurogenin 2
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Probe:
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Math4A cDNA1
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
11-13 somites not shown:
embryonic day 8.5
(more )
Specimen
11-13 somites not shown:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 8.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Structure |
Level |
Pattern |
Image |
Note |
TS13: geniculate placode |
Present |
|
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Expression first detected in geniculate placode (VII) in 11-13 somite embryos.
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Specimen
1A:
embryonic day 9.0
(more )
Specimen
1A:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 9.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
1B:
embryonic day 9.5
(more )
Specimen
1B:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
E9.5 not shown:
embryonic day 9.5
(more )
Specimen
E9.5 not shown:
(close )
Genetic Background:
|
Not Specified
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
|
4% Paraformaldehyde
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Specimen
1C:
embryonic day 10.0
(more )
Specimen
1C:
(close )
Genetic Background:
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Not Specified
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Age:
|
embryonic day 10.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
|
4% Paraformaldehyde
|
Specimen
E10.25 not shown:
embryonic day 10.25
(more )
Specimen
E10.25 not shown:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 10.25
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Sex:
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Not Specified
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Type:
|
whole mount
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Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: geniculate placode |
Absent |
|
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Expression disappeared from geniculate placode around E10.25.
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Specimen
E10.75 not shown:
embryonic day 10.75
(more )
Specimen
E10.75 not shown:
(close )
Genetic Background:
|
Not Specified
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Age:
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embryonic day 10.75
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Sex:
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Not Specified
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Type:
|
whole mount
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Fixation:
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4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: nodose placode |
Absent |
|
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Expression disappeared from petrosal and nodose placode around E10.75.
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TS17: petrosal placode |
Absent |
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Expression disappeared from petrosal and nodose placode around E10.75.
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Specimen
1D:
embryonic day 9.5
(more )
Specimen
1D:
(close )
Genetic Background:
|
Not Specified
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS15: petrosal placode |
Present |
Regionally restricted |
1D
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At E9.5 (24-28 somites), expression in placodal cells and migrating neural precursors immediately after delamination.
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TS15: glossopharyngeal preganglion |
Absent |
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1D
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No expression in precursors near or in ganglionic precursors.
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Specimen
7C:
embryonic day 10.0
(more )
Note: Double labeled: blue - Neurog2; brown - Phox2a (assay MGI:7666609).
Specimen
7C:
(close )
Genetic Background:
|
Not Specified
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Age:
|
embryonic day 10.0
|
Sex:
|
Not Specified
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Type:
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section from whole mount
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Cryosection
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Note:
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Double labeled: blue - Neurog2; brown - Phox2a (assay MGI:7666609).
|
Structure |
Level |
Pattern |
Image |
Note |
TS16: nodose placode |
Present |
Regionally restricted |
7C
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Expression in groups of contiguous placodal cells and migrating precursor cells that have just delaminated.
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TS16: vagus ganglion |
Strong |
Regionally restricted |
7C
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No expression in precursors near or in ganglionic precursors.
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Specimen
7D:
embryonic day 10.0
(more )
Note: Double labeled: blue - Neurog2; brown - Phox2a (assay MGI:7666609).
Specimen
7D:
(close )
Genetic Background:
|
Not Specified
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Age:
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embryonic day 10.0
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: blue - Neurog2; brown - Phox2a (assay MGI:7666609).
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Specimen
7E:
embryonic day 9.5
(more )
Specimen
7E:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Sex:
|
Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
7E':
(close )
Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Phox2atm1Jbr/Phox2atm1Jbr
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
|
whole mount
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Fixation:
|
4% Paraformaldehyde
|
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