Pax3 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:48958 Padilla F, et al., Cadherins M, 11, and 6 expression patterns suggest complementary roles in mouse neuromuscular axis development. Mol Cell Neurosci. 1998 Jul;11(4):217-33
Assay type:	RNA in situ
MGI Accession ID:	MGI:7663998
Gene symbol:	Pax3
Gene name:	paired box 3

Probe:	Pax3 cDNA5
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Results

Specimen 3F: embryonic day 11.5 (more

)
Note: Cross-section from the forelimb level. Double labeled: red - Pax3; blue - Cdh15 (assay MGI:7663986).

Structure	Level	Pattern	Image	Note
TS20: trunk musculature	Present	Regionally restricted	3G , 3F	Expression was detected in distal positions of the body wall. There was no overlap with Cdh15-positive cells.
TS20: limb musculature	Absent		3F

Specimen 3H: embryonic day 11.5 (more

)
Note: Cross-section from the hindlimb level. Double labeled: red - Pax3; blue - Cdh15 (assay MGI:7663986).

Structure	Level	Pattern	Image	Note
TS20: dermomyotome	Present		3H
TS20: hindlimb	Present	Regionally restricted	3H , 3I	Expression was detected in the proximal part of the limb bud. Some labeled cells were intermingled with Cdh15-positive cells, but no doubly stained cells were observed. Expression was also in the distal region of the hindlimb bud.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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