Dnmt3a Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:346060 Huang X, et al., ZFP281 controls transcriptional and epigenetic changes promoting mouse pluripotent state transitions via DNMT3 and TET1. Dev Cell. 2024 Feb 26;59(4):465-481.e6
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7643610
Gene symbol:	Dnmt3a
Gene name:	DNA methyltransferase 3A

Antibody:	anti-Dnmt3a
Detection system:	Secondary antibody

Results

Specimen 2A WT: embryonic day 6.5 (more

)
Note: Triple labeled: green - Dnmt3a; red - Nanog (assay MGI:7643613); blue - Hoechst.

Structure	Level	Pattern	Image	Note
TS8: epiblast	Present		2A WT DNMT3A , 2A WT Merge

Specimen 2A Zfp281-/-: embryonic day 6.5; Zfp281^tm1.1Jlwa/Zfp281^tm1.1Jlwa (more

)
Note: Triple labeled: green - Dnmt3a; red - Nanog (assay MGI:7643613); blue - Hoechst.

Structure	Level	Pattern	Image	Note
TS8: epiblast	Present		2A Zfp281-/- DNMT3A , 2A Zfp281-/- Merge	Expression levels are significantly reduced in the mutant relative to wild type.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 03/25/2025 MGI 6.24