Gene Expression Data
Assay Details
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Assay
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Reference:
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J:290791
Baba Y, et al., Sall1 plays pivotal roles for lens fiber cell differentiation in mouse. Biochem Biophys Res Commun. 2019 May 14;512(4):927-933
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7641981
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Gene symbol:
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Prox1
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Gene name:
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prospero homeobox 1
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Antibody:
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anti-Prox1
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Detection system:
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Secondary antibody coupled to Alexa Fluor 594
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Results
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Specimen
1D:
postnatal day 12; Sall1tm3Ryn/Sall1 +
(more  )
Note: Double labeled: red - Prox1; green - Sall1 reporter (assay MGI:7641977).
Specimen
1D:
(close  )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Sall1tm3Ryn/Sall1+
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Age:
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postnatal day 12
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Prox1; green - Sall1 reporter (assay MGI:7641977).
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Specimen
4A control:
embryonic day 14.5
(more )
Specimen
4A control:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: lens epithelium |
Strong |
Regionally restricted |
4A-1
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Only peripheral region of the lens epithelium showed strong expression.
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| TS22: lens fiber |
Present |
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4A-1
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Specimen
4A gfp/gfp:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Sall1tm3Ryn/Sall1tm3Ryn
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: lens epithelium |
Strong |
Widespread |
4A-2
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Similar as in the control, although strong expression in all region of the lens epithelium.
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| TS22: lens fiber |
Present |
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4A-2
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Specimen
4B control:
embryonic day 17.5
(more )
Specimen
4B control:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
4B gfp/gfp:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Sall1tm3Ryn/Sall1tm3Ryn
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Analysis Tools