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Gene Expression Data
Assay Details
Assay
Reference: J:100439 Cohen S, et al., A semaphorin code defines subpopulations of spinal motor neurons during mouse development. Eur J Neurosci. 2005 Apr;21(7):1767-76
Assay type: RNA in situ
MGI Accession ID: MGI:7615012
Gene symbol: Sema3a
Gene name: sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A
Probe: Sema3a probe
Probe preparation: Antisense RNA
Results
Specimen 1A Brachial: embryonic day 13.5 (more )
Note: Flat-mounted specimen.
Structure Cell Type Level Pattern Image Note
TS21: spinal cord brachial region Present Regionally restricted 1A Sema3A Brachial Expression is in ventral spinal cord cells.
TS21: spinal cord spinal cord motor neuron Present Regionally restricted 1A Sema3A Brachial Expression is in the medial and lateral parts of the median motor column and in subsets of motoneurons in the lateral motor columns.

Specimen 1A Brachial section: embryonic day 13.5 (more )
Structure Cell Type Level Pattern Image Note
TS21: spinal cord brachial region Present Regionally restricted 1A Sema3A Brachial section Expression is in ventral spinal cord cells.
TS21: spinal cord spinal cord motor neuron Present Regionally restricted 1A Sema3A Brachial section Expression is in the medial and lateral parts of the median motor column and in subsets of motoneurons in the lateral motor columns.

Specimen 1A Lumbar: embryonic day 13.5 (more )
Note: Flat-mounted specimen.
Structure Cell Type Level Pattern Image Note
TS21: spinal cord lumbar region spinal cord motor neuron Present Regionally restricted 1A Sema3A Lumbar Expression is in motoneurons subpopulations.

Specimen 2B: embryonic day 13.5 (more )
Note: Double labeled: blue - Sema3a; brown - Etv4 reporter (assay MGI:7615027).
Structure Cell Type Level Pattern Image Note
TS21: spinal cord brachial region spinal cord motor neuron Present Regionally restricted 2B Expression is in a motoneurons subpopulation in the medial part of the lateral motor columns.

Specimen 3C: embryonic day 13.5 (more )
Note: Double labeled: blue - Sema3a; red - Etv1 (assay MGI:7615028).
Structure Cell Type Level Pattern Image Note
TS21: spinal cord lumbar region spinal cord motor neuron Present Regionally restricted 3C Expression is in motoneurons subpopulations.

Specimen 6B: embryonic day 13.5 (more )
Note: Double labeled: blue - Sema3a; brown - Etv4 reporter (assay MGI:7615027).
Structure Cell Type Level Pattern Image Note
TS21: spinal cord brachial region spinal cord motor neuron Present Regionally restricted 6B Expression is in some subpopulations of motoneurons.

Specimen S1: embryonic day 13.5 (more )
Note: Flat-mounted specimen.
Structure Cell Type Level Pattern Image Note
TS21: spinal cord thoracic region spinal cord motor neuron Present Regionally restricted S1_Sema3A Expression is in the lateral part of the median motor column.

Specimen S2 brachial: embryonic day 12.5 (more )
Note: Flat-mounted specimen.
Structure Level Pattern Image Note
TS20: spinal cord brachial region Present S2_Sema3A brachial

Specimen S2 lumbar: embryonic day 12.5 (more )
Note: Flat-mounted specimen.
Structure Level Pattern Image Note
TS20: spinal cord lumbar region Present S2_Sema3A lumbar


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
05/21/2024
MGI 6.23
The Jackson Laboratory