Gene Expression Data
Assay Details
Assay
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Reference:
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J:233793
Taylor-Burds C, et al., Chloride Accumulators NKCC1 and AE2 in Mouse GnRH Neurons: Implications for GABAA Mediated Excitation. PLoS One. 2015;10(6):e0131076
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7612516
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Gene symbol:
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Slc4a2
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Gene name:
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solute carrier family 4 (anion exchanger), member 2
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Antibody:
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anti-AE2
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Detection system:
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Secondary antibody
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Results
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Specimen
2B:
embryonic day 14.5
(more )
Note: Double labeled: red - Slc4a2; green - Gnrh1 (assay MGI:7612496).
Specimen
2B:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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Formaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Slc4a2; green - Gnrh1 (assay MGI:7612496).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS22: brain |
neuron |
Present |
Regionally restricted |
2B2
,
2B1
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Expression in some but not all Gnrh-expressing neurons.
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Specimen
2C:
postnatal day 9-12
(more )
Note: Double labeled: red - Slc4a2; green - Gnrh1 (assay MGI:7612496).
Specimen
2C:
(close )
Genetic Background:
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Not Specified
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Age:
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postnatal day 9-12
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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Formaldehyde
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Embedding:
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Cryosection
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Note:
|
Double labeled: red - Slc4a2; green - Gnrh1 (assay MGI:7612496).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS28: brain |
neuron |
Present |
Regionally restricted |
2C1
,
2C2
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Expression in some but not all Gnrh-expressing neurons.
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Specimen
2D:
postnatal day 9-12
(more )
Note: Double labeled: red - Slc4a2; green - Gnrh1 (assay MGI:7612496).
Specimen
2D:
(close )
Genetic Background:
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Not Specified
|
Age:
|
postnatal day 9-12
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Sex:
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Not Specified
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Type:
|
optical section
|
Fixation:
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Formaldehyde
|
Embedding:
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Cryosection
|
Note:
|
Double labeled: red - Slc4a2; green - Gnrh1 (assay MGI:7612496).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: median eminence |
Present |
Regionally restricted |
2D1
,
2D2
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No expression in Gnrh-expressing fibers.
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Specimen
2E:
postnatal day 9-12
(more )
Note: Double labeled: red - Slc4a2; green - Gfap (assay MGI:7612520).
Specimen
2E:
(close )
Genetic Background:
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Not Specified
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Age:
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postnatal day 9-12
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Sex:
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Not Specified
|
Type:
|
optical section
|
Fixation:
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Formaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Slc4a2; green - Gfap (assay MGI:7612520).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS28: median eminence |
astrocyte |
Present |
Regionally restricted |
2E1
,
2E2
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Expression in Gfap-expressing astocytes.
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