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Gene Expression Data
Assay Details
Assay
Reference: J:233793 Taylor-Burds C, et al., Chloride Accumulators NKCC1 and AE2 in Mouse GnRH Neurons: Implications for GABAA Mediated Excitation. PLoS One. 2015;10(6):e0131076
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7612511
Gene symbol: Slc12a5
Gene name: solute carrier family 12, member 5
Antibody: anti-KCC2
Detection system: Secondary antibody
Results
Specimen 1E3: embryonic day 14.5 (more )
Note: Double labeled: red - Slc12a5; green - Gnrh1 (assay MGI:7612496).
Structure Level Pattern Image Note
TS22: forebrain Present Regionally restricted 1E3 (merged) , 1E3 KCC2 Expression in ventral forebrain.
TS22: head Present Regionally restricted 1E3 (merged) , 1E3 KCC2 No expression in Gnrh-expressing cells at nasal forebrain junction.
Specimen 1F1: postnatal day 9-12 (more )
Note: Double labeled: red - Slc12a5; green - Gnrh1 (assay MGI:7612496).
Structure Cell Type Level Pattern Image Note
TS28: brain Present Widespread 1F1 (upper) , 1F1 (lower) Expression throughtout brain.
TS28: nose neuron Absent 1F1 (upper) , 1F1 (lower) No expression in Gnrh-expressing neurons.
Specimen 1F2: postnatal adult (more )
Note: Double labeled: red - Slc12a5; green - Gnrh1 (assay MGI:7612496).
Structure Cell Type Level Pattern Image Note
TS28: brain Present Widespread 1F2 Expression throughtout brain.
TS28: nose neuron Absent 1F2 No expression in Gnrh-expressing neurons.
Specimen 1G`: postnatal adult (more )
Note: Double labeled: red - Slc12a5; green - Gnrh1 (assay MGI:7612496).
Structure Level Pattern Image Note
TS28: median eminence Present Regionally restricted 1G (left) , 1G (right) No co-localization with Gnrh-expressing fibers.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/21/2024
MGI 6.23 		The Jackson Laboratory