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Gene Expression Data
Assay Details
Assay
Reference: J:233793 Taylor-Burds C, et al., Chloride Accumulators NKCC1 and AE2 in Mouse GnRH Neurons: Implications for GABAA Mediated Excitation. PLoS One. 2015;10(6):e0131076
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7612510
Gene symbol: Slc12a2
Gene name: solute carrier family 12, member 2
Antibody: anti-NKCC1 alphaCT
Detection system: Secondary antibody
Assay notes: Two Slc12a2 were described, but the one used in this assay was not detailed. Antibody Anti-NKCC1 TEFS-2 MGI:MGI:7612461 may have been used in this assay.
Results
Specimen 1A3: embryonic day 14.5 (more )
Note: Double labeled: red - Slc12a2; green - Gnrh1 (assay MGI:7612496).
Structure Cell Type Level Pattern Image Note
TS22: head neuron Present Regionally restricted 1A3 NKCC1 , 1A3 (merged) Expression in Gnrh-expressing neurons in nasal area, with light expression in cell soma.
Specimen 1B1: postnatal day 9-12 (more )
Note: Double labeled: red - Slc12a2; green - Gnrh1 (assay MGI:7612496).
Structure Cell Type Level Pattern Image Note
TS28: preoptic area neuron Present Regionally restricted 1B1 (upper) , 1B1 (lower) Expression surrounding pre-optic tissue. Expression co-localized in Gnrh expressing neurons in pre-pubertal mice. Expression most intense in pre-pubertal mice.
Specimen 1B2: postnatal adult (more )
Note: Double labeled: red - Slc12a2; green - Gnrh1 (assay MGI:7612496).
Structure Cell Type Level Pattern Image Note
TS28: brain neuron Present Regionally restricted 1B2 Expression co-localized in Gnrh expressing neurons in adult mice. Very low level of expression throughout brain.
Specimen 1C: postnatal adult (more )
Note: Double labeled: red - Slc12a2; green - Gnrh1 (assay MGI:7612496).
Structure Level Pattern Image Note
TS28: median eminence Present Regionally restricted 1C (right) , 1C (left) Expression in median eminence not co-localized with Gnrh-expressing fibers.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/21/2024
MGI 6.23 		The Jackson Laboratory