Probe:	Rgs4 cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS22: midbrain	Present	Regionally restricted	4A' , 4A	Expressed in the dorsal region, directly adjacent and dorsal to the TH-positive area, with several cells co-localised with TH in the dorsal TH expression domain.

Structure	Level	Pattern	Image	Note
TS22: midbrain	Present	Regionally restricted	4B , 4B'	Expressed in the dorsal region, directly adjacent and dorsal to the TH-positive area, with several cells co-localised with TH in the dorsal TH expression domain.
TS22: prosomere 1	Present		4B

Structure	Level	Pattern	Image	Note
TS22: midbrain	Present	Regionally restricted	4E , 4E'	Expressed in the dorsal mesodiencephalic dopamine progenitor field. Most of them were adjacent to TH-positive cells; however, some cells clearly colocalized with TH.

Structure	Level	Pattern	Image	Note
TS22: midbrain	Present	Regionally restricted	4F' , 4F	Expressed in the dorsal mesodiencephalic dopamine progenitor field. Most of them were adjacent to TH-positive cells; however, some cells clearly colocalized with TH.

Structure	Level	Pattern	Image	Note
TS22: midbrain	Present		5OO
TS22: hindbrain	Present	Regionally restricted	5OO	Expressed in a small group of cells.

Structure	Level	Pattern	Image	Note
TS22: midbrain	Present		5PP" , 5PP' , 5PP
TS22: hindbrain	Present	Regionally restricted	5PP	Expressed in a small group of cells.

Structure	Level	Pattern	Image	Note
TS22: midbrain	Present		5QQ
TS22: hindbrain	Present	Regionally restricted	5QQ	Expressed in a small group of cells.

Structure	Level	Pattern	Image	Note
TS22: midbrain	Present		5RR' , 5RR
TS22: hindbrain	Present	Regionally restricted	5RR	Expressed in a small group of cells.

Structure	Level	Pattern	Image	Note
TS22: midbrain	Present		5SS
TS22: hindbrain	Present	Regionally restricted	5SS	Expressed in a small group of cells.

Structure	Level	Pattern	Image	Note
TS22: midbrain	Present		5TT	Decreased expression compared with the wild-type.
TS22: hindbrain	Present	Regionally restricted	5TT	Expressed in a small group of cell, similar as in the wild-type.
TS22: prosomere 3	Present		5TT	A dramatic loss of expression was observed.
TS22: hypothalamus	Present	Regionally restricted	5TT	A dramatic loss of expression was observed in the ventral hypothalamic area.

Structure	Level	Pattern	Image	Note
TS22: midbrain	Present		5UU , 5UU' , 5UU"	Decreased expression compared with the wild-type. The most obvious reduction was in the rostral domain located directly dorsal to the mesodiencephalic dopamine neuronal area.
TS22: hindbrain	Present		5UU	Expressed in a small group of cell, similar as in the wild-type.
TS22: prosomere 3	Present		5UU	A dramatic loss of expression was observed.
TS22: hypothalamus	Present	Regionally restricted	5UU	A dramatic loss of expression was observed in the ventral hypothalamic area.

Structure	Level	Pattern	Image	Note
TS22: midbrain	Present		5VV	Decreased expression compared with the wild-type.
TS22: hindbrain	Present		5VV	Expressed in a small group of cell, similar as in the wild-type.
TS22: prosomere 3	Present		5VV	A dramatic loss of expression was observed.
TS22: hypothalamus	Present	Regionally restricted	5VV	A dramatic loss of expression was observed in the ventral hypothalamic area.

Structure	Level	Pattern	Image	Note
TS22: midbrain	Present		5WW , 5WW'	Decreased expression compared with the wild-type. The most obvious reduction was in the rostral domain located directly dorsal to the mesodiencephalic dopamine neuronal area.
TS22: hindbrain	Present		5WW	Expressed in a small group of cell, similar as in the wild-type.

Structure	Level	Pattern	Image	Note
TS22: midbrain	Present		5XX	Decreased expression compared with the wild-type.
TS22: hindbrain	Present		5XX	Expressed in a small group of cell, similar as in the wild-type.

Structure	Level	Pattern	Image	Note
TS22: midbrain ventricular layer	Present	Regionally restricted		Highly and specifically expressed in a set of cells, located around the alar plate and basal plate.