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Gene Expression Data
Assay Details
Assay
Reference: J:336871 Toonen JA, et al., A Disintegrin and Metalloproteinase10 (ADAM10) Regulates NOTCH Signaling during Early Retinal Development. PLoS One. 2016;11(5):e0156184
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7587743
Gene symbol: Isl1
Gene name: ISL1 transcription factor, LIM/homeodomain
Antibody: anti-ISL1
Detection system: Secondary antibody coupled to Alexa Fluor 546
Results
Specimen 4G/J: embryonic day 13.5; Adam10tm1Zhu/? (more )
Note: Triple labeled: red - Isl1; blue - Otx2 (assay MGI:7587742); green - EdU.
Structure Cell Type Level Pattern Image Note
TS21: neural retinal epithelium retinal ganglion cell Present Regionally restricted 4G , 4J Expression in early ganglion cells. Expression in basal layer.
Specimen 4H/K: embryonic day 13.5; Adam10tm1Zhu/Adam10tm1Zhu, Tg(Six3-cre)69Frty/0 (more )
Note: Conditional mutant. Triple labeled: red - Isl1; blue - Otx2 (assay MGI:7587742); green - EdU.
Structure Level Pattern Image Note
TS21: neural retinal epithelium Present Widespread 4K , 4H Expression in all layers in cells that were highly disorganized.
Specimen 5C: embryonic day 16.5; Adam10tm1Zhu/? (more )
Note: Double labeled: red - Isl1; green - Otx2 (assay MGI:7587742).
Structure Cell Type Level Pattern Image Note
TS24: neural retina retinal ganglion cell Present Regionally restricted 5C Expression in ganglion cells at basal surface.
Specimen 5D: embryonic day 16.5; Adam10tm1Zhu/Adam10tm1Zhu, Tg(Six3-cre)69Frty/0 (more )
Note: Conditional mutant. Double labeled: red - Isl1; green - Otx2 (assay MGI:7587742).
Structure Level Pattern Image Note
TS24: neural retina Present Scattered 5D Scattered expression through entire retina, not associated with rosettes.
Specimen S4A: embryonic day 13.5; Gt(ROSA)26Sortm1(Notch1)Dam/Gt(ROSA)26Sor+, Tg(Six3-cre)69Frty/0 (more )
Note: Conditional mutant. Triple labeled: red - Isl1; blue - Otx2 (assay MGI:7587742); green - EdU.
Structure Level Pattern Image Note
TS21: neural retinal epithelium Present Regionally restricted S4A No expressing cells within central regions.
Specimen S4B: embryonic day 13.5; Adam10tm1Zhu/Adam10tm1Zhu, Gt(ROSA)26Sortm1(Notch1)Dam/Gt(ROSA)26Sor+, Tg(Six3-cre)69Frty/0 (more )
Note: Conditional mutant. Triple labeled: red - Isl1; blue - Otx2 (assay MGI:7587742); green - EdU.
Structure Level Pattern Image Note
TS21: neural retinal epithelium Present Regionally restricted S4B No expressing cells within central regions.
Specimen S4C: embryonic day 13.5; Gt(ROSA)26Sortm1(Notch1)Dam/Gt(ROSA)26Sor+, Tg(Six3-cre)69Frty/0 (more )
Note: Conditional mutant. Triple labeled: red - Isl1; blue - Otx2 (assay MGI:7587742); green - EdU.
Structure Level Pattern Image Note
TS21: neural retinal epithelium Present Regionally restricted S4C Very few expressing cells at periphery of retina.
Specimen S4D: embryonic day 13.5; Adam10tm1Zhu/Adam10tm1Zhu, Gt(ROSA)26Sortm1(Notch1)Dam/Gt(ROSA)26Sor+, Tg(Six3-cre)69Frty/0 (more )
Note: Conditional mutant. Triple labeled: red - Isl1; blue - Otx2 (assay MGI:7587742); green - EdU.
Structure Level Pattern Image Note
TS21: neural retinal epithelium Present Regionally restricted S4D Very few expressing cells at periphery of retina.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
MGI 6.24 		The Jackson Laboratory