Antibody:

anti-Tmod1 (R1749)

Structure	Level	Pattern	Image	Note
TS13: heart	Present		2C Tmod

Structure	Level	Pattern	Image	Note
TS17: somite	Weak

Structure	Cell Type	Level	Pattern	Image	Note
TS12: cardiac muscle tissue	cardiac muscle cell	Present		6_E8-8.5 merge , 6_E8-8.5 Tmod1	In cardiac myocytes staining was frequently concentrated between periodic dots of sarcomeric alpha-actinin (Actn2), suggesting Tmod1 association with thin filament pointed ends between I-Z-I complexes in nascent myofibrils.
TS12: heart		Present	Diffuse	6_E8-8.5 Tmod1 , 6_E8-8.5 merge

Structure	Cell Type	Level	Pattern	Image	Note
TS15: cardiac muscle tissue	cardiac muscle cell	Present		6_E9.5 Tmod1 , 6_E9.5 merge	Immunopositive striations became more defined in the center of sarcomeres of many myofibrils, alternating with Actn2 striations.
TS15: heart		Weak	Diffuse	6_E9.5 Tmod1 , 6_E9.5 merge

Structure	Cell Type	Level	Pattern	Image	Note
TS20: cardiac muscle tissue	cardiac muscle cell	Present		6_E12.5 Tmod1 , 6_E12.5 merge	Immunopositive striations were organized in a relatively mature pattern.

Structure	Cell Type	Level	Pattern	Image	Note
TS12: cardiac muscle tissue	cardiac muscle cell	Present		7_E8-8.5 Tmod1 top , 7_E8-8.5 merge top	In E8-8.5 embryos immunoreactivity was associated with many types of F-actin-containing fibrillar structures. Staining was frequently discontinuous and was concentrated in broad bands that often coincided with decreases or discontinuities in F-actin staining.

Structure	Cell Type	Level	Pattern	Image	Note
TS12: cardiac muscle tissue	cardiac muscle cell	Present		7_E8-8.5 merge bottom , 7_E8-8.5 Tmod1 bottom	In E8-8.5 embryos immunoreactivity was associated with many types of F-actin-containing fibrillar structures. Staining was frequently discontinuous and was concentrated in broad bands that often coincided with decreases or discontinuities in F-actin staining.

Structure	Cell Type	Level	Pattern	Image	Note
TS12: heart	cardiac muscle cell	Present		7_E8-8.5 inset merge , 7_E8-8.5 inset Tmod1	In sarcomeres with clear gaps in F-actin staining, the Tmod1-stained bands were often broad and poorly defined.

Structure	Cell Type	Level	Pattern	Image	Note
TS15: heart	cardiac muscle cell	Present		7_E9.5 Tmod1 , 7_E9.5 merge	In E9.5 embryos, the Tmod1-positive striations were sharper and better defined, similar to Tmod1 in mature myofibrils.