About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:143529 Ichise H, et al., Phospholipase C{gamma}2 is necessary for separation of blood and lymphatic vasculature in mice. Development. 2009 Jan;136(2):191-5
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:7574221
Gene symbol: Plcg2
Gene name: phospholipase C, gamma 2
Reporter: GFP
Antibody: anti-GFP (Nacalai)
Detection system: Secondary antibody
Results
Specimen 3A: embryonic day 14.5; Plcg2tm1Ichi/Plcg2tm1Ichi (more )
Note: Double labeled: green - Plcg2 reporter; red - Adgre1 (assay MGI:7574224).
Structure Cell Type Level Pattern Image Note
TS22: blood monocyte Present 3A left , 3A right Coexpressed with monocyte marker F4/80 (Adgre1).
Specimen 3B: embryonic day 13.5; Plcg2tm1Ichi/Plcg2tm1Ichi (more )
Note: Double labeled: green - Plcg2 reporter; red - Pecam1 (assay MGI:7574218).
Structure Cell Type Level Pattern Image Note
TS21: blood vessel endothelium vein endothelial cell Present Regionally restricted 3B left , 3B right Expressed in some cells.
TS21: blood platelet Present 3B left , 3B right Expressed in platelets lack nuclei.
Specimen 3C: embryonic day 14.5; Plcg2tm1Ichi/Plcg2tm1Ichi (more )
Note: Double labeled: green - Plcg2 reporter; red - Pecam1 (assay MGI:7574218).
Structure Level Pattern Image Note
TS22: blood vessel endothelium Present Regionally restricted 3C right , 3C left Expressed in a subpopulation.
Specimen 3D +/EGFP: embryonic day 15.5; Plcg2tm1Ichi/Plcg2+ (more )
Note: Double labeled: green - Plcg2 reporter; red - Lyve1 (assay MGI:7574220).
Structure Cell Type Level Pattern Image Note
TS23: lymphatic vessel endothelium endothelial cell of lymphatic vessel Absent 3D +/EGFP right , 3D +/EGFP left
TS23: blood monocyte Present 3D +/EGFP left , 3D +/EGFP right
Specimen 3D EGFP/EGFP: embryonic day 15.5; Plcg2tm1Ichi/Plcg2tm1Ichi (more )
Note: Double labeled: green - Plcg2 reporter; red - Lyve1 (assay MGI:7574220).
Structure Cell Type Level Pattern Image Note
TS23: lymphatic vessel endothelium endothelial cell of lymphatic vessel Absent 3D EGFP/EGFP right , 3D EGFP/EGFP left
Specimen 3Ea/c: embryonic day 13.5; Plcg2tm1Ichi/Plcg2+ (more )
Note: Double labeled: green - Plcg2 reporter; red - Pecam1 (assay MGI:7574218).
Structure Cell Type Level Pattern Image Note
TS21: artery endothelium endothelial cell of artery Present 3Ea , 3Ec Expressed predominantly in arterial endothelial cells.
TS21: blood vessel endothelium vein endothelial cell Trace 3Ec , 3Ea Rarely in venous endothelial cells.
TS21: lymph sac endothelial cell of lymphatic vessel Absent 3Ec , 3Ea
Specimen 3Eb/d: embryonic day 13.5; Plcg2tm1Ichi/Plcg2tm1Ichi (more )
Note: Double labeled: green - Plcg2 reporter; red - Pecam1 (assay MGI:7574218).
Structure Cell Type Level Pattern Image Note
TS21: artery endothelium endothelial cell of artery Present 3Eb , 3Ed Expressed predominantly in arterial endothelial cells.
TS21: blood vessel endothelium vein endothelial cell Trace 3Ed , 3Eb Rarely in venous endothelial cells.
TS21: lymph sac endothelial cell of lymphatic vessel Absent 3Ed , 3Eb
Specimen not shown: embryonic day 14.5; Plcg2tm1Ichi/Plcg2tm1Ichi (more )
Structure Cell Type Level Pattern Image Note
TS22: blood vessel smooth muscle blood vessel smooth muscle cell Absent
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
05/07/2024
MGI 6.23 		The Jackson Laboratory