Gene Expression Data
Assay Details
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Assay
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Reference:
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J:119027
Inman KE, et al., Brachyury is required for elongation and vasculogenesis in the murine allantois. Development. 2006 Aug;133(15):2947-59
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7571423
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Gene symbol:
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Pecam1
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Gene name:
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platelet/endothelial cell adhesion molecule 1
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Antibody:
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MEC 13.3
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Detection system:
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Secondary antibody coupled to horseradish peroxidase
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Results
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Specimen
6Aa:
embryonic day 8.0; Kdrtm1Jrt/ Kdrtm1Jrt
(more  )
Note: Early headfold stage embryo. Double labeled: brown - Pecam1; blue - Kdr reporter (assay MGI:7571422).
Specimen
6Aa:
(close  )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Kdrtm1Jrt/Kdrtm1Jrt
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Age:
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embryonic day 8.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Early headfold stage embryo. Double labeled: brown - Pecam1; blue - Kdr reporter (assay MGI:7571422).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: allantois |
Present |
Regionally restricted |
6Aa
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Expression is in a central vessel that extended distally and proximally.
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| TS12: extraembryonic blood vessel |
Present |
Regionally restricted |
6Aa
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Expression is in a central vessel that extended distally and proximally.
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Specimen
6Aa inset:
embryonic day 7.5; Kdrtm1Jrt/ Kdrtm1Jrt
(more )
Note: Late bud stage embryo. Double labeled: brown - Pecam1; blue - Kdr reporter (assay MGI:7571422).
Specimen
6Aa inset:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Kdrtm1Jrt/Kdrtm1Jrt
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Late bud stage embryo. Double labeled: brown - Pecam1; blue - Kdr reporter (assay MGI:7571422).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: allantois |
Present |
Regionally restricted |
6Aa inset
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Expression is in a small cluster.
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Specimen
6Ab:
embryonic day 8.0; Kdrtm1Jrt/ Kdrtm1Jrt
(more )
Note: 2-somite stage embryo. Double labeled: brown - Pecam1; blue - Kdr reporter (assay MGI:7571422).
Specimen
6Ab:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Kdrtm1Jrt/Kdrtm1Jrt
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Age:
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embryonic day 8.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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2-somite stage embryo. Double labeled: brown - Pecam1; blue - Kdr reporter (assay MGI:7571422).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: allantois |
Present |
Regionally restricted |
6Ab
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Expression is in a central vessel that extended distally and proximally, and converged upon the developing embryonic dorsal aortae.
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| TS12: extraembryonic blood vessel |
Present |
Regionally restricted |
6Ab
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Expression is in a central vessel that extended distally and proximally, and converged upon the developing embryonic dorsal aortae.
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| TS12: dorsal aorta |
Present |
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6Ab
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Specimen
6Ac:
embryonic day 8.5; Kdrtm1Jrt/ Kdrtm1Jrt
(more )
Note: 6-somite stage embryo. Double labeled: brown - Pecam1; blue - Kdr reporter (assay MGI:7571422).
Specimen
6Ac:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Kdrtm1Jrt/Kdrtm1Jrt
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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6-somite stage embryo. Double labeled: brown - Pecam1; blue - Kdr reporter (assay MGI:7571422).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: allantois blood vessel |
Present |
Regionally restricted |
6Ac
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Expression is in a central vessel and its branches that extended distally and proximally, and converged upon the developing embryonic dorsal aortae.
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| TS13: blood vessel |
Present |
Regionally restricted |
6Ac
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Expression is in a central vessel and its branches that extended distally and proximally, and converged upon the developing embryonic dorsal aortae.
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| TS13: dorsal aorta |
Present |
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6Ac
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Specimen
6Ba:
embryonic day 8.0
(more )
Note: 2-somite stage embryo.
Specimen
6Ba:
(close )
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Genetic Background:
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involves: 101/H * C3H/HeH * STOCK-Tc/J
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Age:
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embryonic day 8.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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2-somite stage embryo.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: allantois |
Present |
Regionally restricted |
6Ba
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Expression is in a central vessel that extended distally and proximally, and converged upon the developing embryonic dorsal aortae.
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| TS12: extraembryonic blood vessel |
Present |
Regionally restricted |
6Ba
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Expression is in a central vessel that extended distally and proximally, and converged upon the developing embryonic dorsal aortae.
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| TS12: dorsal aorta |
Present |
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6Ba
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Specimen
6Bb:
embryonic day 8.0; Tc/T +
(more )
Note: 2-somite stage embryo.
Specimen
6Bb:
(close )
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Genetic Background:
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involves: 101/H * C3H/HeH * STOCK-Tc/J
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Mutant Allele(s):
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Tc/T+
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Age:
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embryonic day 8.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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2-somite stage embryo.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: allantois |
Present |
Regionally restricted |
6Bb
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Expression in a central allantoic vessel that extended distally and proximally, and converged upon the developing embryonic dorsal aortae was variable in this mutant.
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| TS12: extraembryonic blood vessel |
Present |
Regionally restricted |
6Bb
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Expression in a central allantoic vessel that extended distally and proximally, and converged upon the developing embryonic dorsal aortae was variable in this mutant.
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| TS12: embryo |
Present |
Regionally restricted |
6Bb
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Absent in the posterior embryonic region.
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| TS12: dorsal aorta |
Absent |
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6Bb
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Specimen
6Bc:
embryonic day 8.0; Tc/T +
(more )
Note: 2-somite stage embryo.
Specimen
6Bc:
(close )
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Genetic Background:
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involves: 101/H * C3H/HeH * STOCK-Tc/J
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Mutant Allele(s):
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Tc/T+
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Age:
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embryonic day 8.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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2-somite stage embryo.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: allantois |
Present |
Regionally restricted |
6Bc
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Expression in a central allantoic vessel that extended distally and proximally, and converged upon the developing embryonic dorsal aortae was variable in this mutant.
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| TS12: extraembryonic blood vessel |
Present |
Regionally restricted |
6Bc
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Expression in a central allantoic vessel that extended distally and proximally, and converged upon the developing embryonic dorsal aortae was variable in this mutant.
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| TS12: embryo |
Present |
Regionally restricted |
6Bc
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Absent in the posterior embryonic region.
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| TS12: dorsal aorta |
Absent |
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6Bc
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Specimen
6Bd:
embryonic day 8.0; Tc/ Tc
(more )
Note: 2-somite stage embryo.
Specimen
6Bd:
(close )
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Genetic Background:
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involves: 101/H * C3H/HeH * STOCK-Tc/J
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Mutant Allele(s):
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Tc/Tc
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Age:
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embryonic day 8.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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2-somite stage embryo.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: allantois |
Present |
Single cells |
6Bd
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A single positive cell was detetcted in this mutant.
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| TS12: embryo |
Present |
Regionally restricted |
6Bd
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Absent in the posterior embryonic region.
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Analysis Tools