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Gene Expression Data
Assay Details
Assay
Reference: J:73287 Chow RL, et al., Vsx1, a rapidly evolving paired-like homeobox gene expressed in cone bipolar cells. Mech Dev. 2001 Dec;109(2):315-22
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7565338
Gene symbol: Vsx1
Gene name: visual system homeobox 1
Antibody: anti-Vsx1
Detection system: Secondary antibody coupled to Cy3
Results
Specimen 5A/C: postnatal month 5 (more )
Note: Double labeled: red - Vsx1; green - Rcvrn (assay MGI:7565339).
Structure Cell Type Level Pattern Image Note
TS28: retina inner nuclear layer OFF-bipolar cell Present 5A , 5C Coexpressed with recoverin.
TS28: retina inner nuclear layer ON-bipolar cell Present 5A , 5C Coexpressed with recoverin.
TS28: retina inner nuclear layer cone retinal bipolar cell Present 5A , 5C Also expressed in recoverin-negative cone bipolar cells.
Specimen 5D/F: postnatal month 5 (more )
Note: Double labeled: red - Vsx1; green - PKC.
Structure Cell Type Level Pattern Image Note
TS28: retina inner nuclear layer Present 5D , 5F
TS28: retina inner nuclear layer rod bipolar cell Absent 5D , 5F Not colocalize with the rod bipolar cell marker PKC.
Specimen 5G/I: postnatal month 5 (more )
Note: Double labeled: red - Vsx1; green - Stx1a (assay MGI:7565340).
Structure Cell Type Level Pattern Image Note
TS28: retina inner nuclear layer Present 5I , 5G
TS28: retina inner nuclear layer amacrine cell Absent 5I , 5G No colocalization with the pan-amacrine marker Stx1a/HPC-1.
Specimen 5J/L: postnatal month 5 (more )
Note: Double labeled: red - Vsx1; green - Calb1 (assay MGI:7565341).
Structure Cell Type Level Pattern Image Note
TS28: retina inner nuclear layer Present 5L , 5J
TS28: retina inner nuclear layer amacrine cell Absent 5J , 5L No colocalization with the horizontal and amacrine marker Calb1.
TS28: retina inner nuclear layer retina horizontal cell Absent 5J , 5L No colocalization with the horizontal and amacrine marker Calb1.
Specimen 5M/O: postnatal month 5 (more )
Note: Double labeled: red - Vsx1; green - Ccnd3 (assay MGI:7565342).
Structure Cell Type Level Pattern Image Note
TS28: retina inner nuclear layer Present 5O , 5M
TS28: retina inner nuclear layer Mueller cell Absent 5M , 5O No colocalization with the Muller cell marker Ccnd3.
Specimen not shown: postnatal day 5 (more )
Structure Level Pattern Image Note
TS28: retina inner nuclear layer Present
TS28: ciliary body Absent
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
MGI 6.23 		The Jackson Laboratory