Sox2 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:336228 Thompson MJ, et al., Early radial positional information in the cochlea is optimized by a precise linear BMP gradient and enhanced by SOX2. Sci Rep. 2023 May 26;13(1):8567
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7547360
Gene symbol:	Sox2
Gene name:	SRY (sex determining region Y)-box 2

Antibody:	anti-Sox2 (AF2018)
Detection system:	Secondary antibody coupled to Alexa Fluor

Results

1A ©

Specimen 1A: embryonic day 12.5 (more

)
Note: Double labeled: green - Sox2; orange - Smad1 (assay MGI:7547365), Smad5 (assay MGI:7547366), and Smad9 (assay MGI:7547367).

Structure	Level	Pattern	Image	Note
TS20: cochlear duct	Present	Regionally restricted	1A	Expression is seen in the prosensory domain.

1B ©

Specimen 1B: embryonic day 13.5 (more

)
Note: Double labeled: green - Sox2; orange - Smad1 (assay MGI:7547365), Smad5 (assay MGI:7547366), and Smad9 (assay MGI:7547367).

Structure	Level	Pattern	Image	Note
TS21: cochlear duct	Present	Regionally restricted	1B	Expression is seen in the prosensory domain.

1C ©

Specimen 1C: embryonic day 14.5 (more

)
Note: Triple labeled: white - Sox2; green - Myo6 (assay MGI:7547362); red - phalloidin.

Structure	Level	Pattern	Image	Note
TS22: cochlear duct	Present	Regionally restricted	1C	Expression is seen in the prosensory domain.

2B ©

Specimen 2B: embryonic day 12.5 (more

)
Note: Double labeled: green - Sox2; orange - Smad1 (assay MGI:7547365), Smad5 (assay MGI:7547366), and Smad9 (assay MGI:7547367).

Structure	Level	Pattern	Image	Note
TS20: cochlear duct	Present	Regionally restricted	2B	Expression is seen in the prosensory domain.

S1A ©

Specimen S1A: embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: cochlear duct	Present	Regionally restricted	S1A	Expression is seen in the prosensory domain.

S1E ©

Specimen S1E: embryonic day 13.5 (more

)

Structure	Level	Pattern	Image	Note
TS21: cochlear duct	Present	Regionally restricted	S1E	Expression is seen in the prosensory domain.

S1I ©

Specimen S1I: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS22: cochlear duct	Present	Regionally restricted	S1I	Expression is seen in the prosensory domain.

S3A ©

Specimen S3A: embryonic day 13.5 (more

)
Note: Control. Triple labeled: red - Sox2; green - Jag1 (assay MGI:7547359); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS21: cochlear duct	Present	Regionally restricted	S3A	Expression is seen in the prosensory domain.

S3B ©

Specimen S3B: embryonic day 13.5; Isl1^tm1(cre)Sev/Isl1⁺, Smad4^tm2.1Cxd/Smad4^tm2.1Cxd (more

)
Note: Conditional mutant. Triple labeled: red - Sox2; green - Jag1 (assay MGI:7547359); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS21: cochlear duct	Present		S3B	Expression is expanded laterally in the mutant.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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