About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:273130 Ding W, et al., Cenpj Regulates Cilia Disassembly and Neurogenesis in the Developing Mouse Cortex. J Neurosci. 2019 Mar 13;39(11):1994-2010
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7547340
Gene symbol: Tubg1
Gene name: tubulin, gamma 1
Antibody: anti-gamma-tubulin (T3559)
Detection system: Secondary antibody coupled to Alexa Fluor
Assay notes: Two gamma-tubulin antibodies were described, but the one used in this assay was not detailed. Anti-gamma-tubulin (T5326) name MGI:7547289 may have been used in this assay.
Results
Specimen 3A gamma-tubulin Cenpj+/+;CiliaGFP/+ (upper): embryonic day 15.5; Gt(ROSA)26Sortm1.1(Sstr3/GFP)Bky/Gt(ROSA)26Sor+ (more )
Note: Double labeled: red - Tubg1; green - GFP reporter.
Structure Cell Type Level Pattern Image Note
TS23: telencephalon ventricular layer radial glial cell Present Regionally restricted 3A Cenpj+/+CiliaGFP (upper)
Specimen 3A gamma-tubulin CenpjCKO;CiliaGFP/+ (upper): embryonic day 15.5; Cpaptm1c(EUCOMM)Wtsi/Cpaptm1c(EUCOMM)Wtsi, Emx1tm1(cre)Krj/Emx1+, Gt(ROSA)26Sortm1.1(Sstr3/GFP)Bky/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Double labeled: red - Tubg1; green - GFP reporter.
Structure Cell Type Level Pattern Image Note
TS23: telencephalon ventricular layer radial glial cell Present Regionally restricted 3A CenpjCKOCiliaGFP (upper) Expression in radial glial cells on lateral ventricle surface.
Specimen 3A gamma-tubulin Cenpj+/+;CiliaGFP/+ (lower): embryonic day 15.5; Gt(ROSA)26Sortm1.1(Sstr3/GFP)Bky/Gt(ROSA)26Sor+ (more )
Note: Double labeled: red - Tubg1; green - GFP reporter.
Structure Cell Type Level Pattern Image Note
TS23: telencephalon ventricular layer radial glial cell Present 3A Cenpj+/+CiliaGFP (lower) Expression in radial glial cells on lateral ventricle surface.
Specimen 3A gamma-tubulin CenpjCKO;CiliaGFP/+ (lower): embryonic day 15.5; Cpaptm1c(EUCOMM)Wtsi/Cpaptm1c(EUCOMM)Wtsi, Emx1tm1(cre)Krj/Emx1+, Gt(ROSA)26Sortm1.1(Sstr3/GFP)Bky/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Double labeled: red - Tubg1; green - GFP reporter.
Structure Cell Type Level Pattern Image Note
TS23: telencephalon ventricular layer radial glial cell Present Regionally restricted 3A CenpjCKOCiliaGFP (lower) Expression in radial glial cells on lateral ventricle surface.
Specimen 5J gamma-tubulin Cenpj+/+: postnatal adult (more )
Note: Double labeled: red - Tubg1; green - Tjp1 (assay MGI:7547338).
Structure Level Pattern Image Note
TS28: brain ventricular layer Present Regionally restricted 5J Cempj+/+ Expression in basal bodies, the majority of which were in the same direction.
Specimen 5J gamma-tubulin CenpjCKO: postnatal adult; Cpaptm1c(EUCOMM)Wtsi/Cpaptm1c(EUCOMM)Wtsi, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant. Double labeled: red - Tubg1; green - Tjp1 (assay MGI:7547338).
Structure Level Pattern Image Note
TS28: brain ventricular layer Present Regionally restricted 5J CenpjCKO Basal body patches often migrated in different direction from neighboring cells.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
03/25/2025
MGI 6.24 		The Jackson Laboratory